Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs

Zorana Milanović, Jelena Vekić, Vladimir Radonjić, Anja Ilić Božović, Aleksandra Zeljković, Jelena Janac, Vesna Spasojević-Kalimanovska, Jesse Buch, Ramaswamy Chandrashekar, Žanka Bojić-Trbojević, Ljiljana Hajduković, Mary M Christopher, Milica Kovačević Filipović

Research output: Contribution to journalArticle

Abstract

Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P <.001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P <.001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.

Original languageEnglish (US)
JournalJournal of Veterinary Internal Medicine
DOIs
StatePublished - Jan 1 2019

Fingerprint

Babesia canis
Babesia
apoproteins
Apoproteins
Serum Amyloid A Protein
lipoproteins
blood lipids
Lipoproteins
amyloid
Acute-Phase Reaction
Apolipoprotein A-I
Dogs
Lipids
dogs
Infection
Serum
infection
high density lipoprotein
Cholesterol
cholesterol

Keywords

  • acute phase response
  • apolipoprotein A-1
  • high-density lipoprotein
  • lipoprotein diameter
  • serum amyloid A

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Milanović, Z., Vekić, J., Radonjić, V., Ilić Božović, A., Zeljković, A., Janac, J., ... Kovačević Filipović, M. (2019). Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. Journal of Veterinary Internal Medicine. https://doi.org/10.1111/jvim.15537

Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. / Milanović, Zorana; Vekić, Jelena; Radonjić, Vladimir; Ilić Božović, Anja; Zeljković, Aleksandra; Janac, Jelena; Spasojević-Kalimanovska, Vesna; Buch, Jesse; Chandrashekar, Ramaswamy; Bojić-Trbojević, Žanka; Hajduković, Ljiljana; Christopher, Mary M; Kovačević Filipović, Milica.

In: Journal of Veterinary Internal Medicine, 01.01.2019.

Research output: Contribution to journalArticle

Milanović, Z, Vekić, J, Radonjić, V, Ilić Božović, A, Zeljković, A, Janac, J, Spasojević-Kalimanovska, V, Buch, J, Chandrashekar, R, Bojić-Trbojević, Ž, Hajduković, L, Christopher, MM & Kovačević Filipović, M 2019, 'Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs', Journal of Veterinary Internal Medicine. https://doi.org/10.1111/jvim.15537
Milanović, Zorana ; Vekić, Jelena ; Radonjić, Vladimir ; Ilić Božović, Anja ; Zeljković, Aleksandra ; Janac, Jelena ; Spasojević-Kalimanovska, Vesna ; Buch, Jesse ; Chandrashekar, Ramaswamy ; Bojić-Trbojević, Žanka ; Hajduković, Ljiljana ; Christopher, Mary M ; Kovačević Filipović, Milica. / Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. In: Journal of Veterinary Internal Medicine. 2019.
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abstract = "Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P <.001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5{\%}, 27.7{\%}-93.5{\%} versus 89.2{\%}, 75.1{\%}-93.5{\%}) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P <.001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.",
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author = "Zorana Milanović and Jelena Vekić and Vladimir Radonjić and {Ilić Božović}, Anja and Aleksandra Zeljković and Jelena Janac and Vesna Spasojević-Kalimanovska and Jesse Buch and Ramaswamy Chandrashekar and Žanka Bojić-Trbojević and Ljiljana Hajduković and Christopher, {Mary M} and {Kovačević Filipović}, Milica",
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T1 - Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs

AU - Milanović, Zorana

AU - Vekić, Jelena

AU - Radonjić, Vladimir

AU - Ilić Božović, Anja

AU - Zeljković, Aleksandra

AU - Janac, Jelena

AU - Spasojević-Kalimanovska, Vesna

AU - Buch, Jesse

AU - Chandrashekar, Ramaswamy

AU - Bojić-Trbojević, Žanka

AU - Hajduković, Ljiljana

AU - Christopher, Mary M

AU - Kovačević Filipović, Milica

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P <.001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P <.001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.

AB - Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P <.001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P <.001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.

KW - acute phase response

KW - apolipoprotein A-1

KW - high-density lipoprotein

KW - lipoprotein diameter

KW - serum amyloid A

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