Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST

R. M. Buckley, R. A. Grahn, B. Gandolfi, J. R. Herrick, M. D. Kittleson, H. L. Bateman, J. Newsom, W. F. Swanson, D. J. Prieur, L. A. Lyons

Research output: Contribution to journalArticle

Abstract

Chediak-Higashi Syndrome (CHS) is a well-characterized, autosomal recessively inherited lysosomal disease caused by mutations in lysosomal trafficking regulator (LYST). The feline model for CHS was originally maintained for ~20 years. However, the colonies were disbanded and the CHS cat model was lost to the research community before the causative mutation was identified. To resurrect the cat model, semen was collected and cryopreserved from a lone, fertile, CHS carrier male. Using cryopreserved semen, laparoscopic oviductal artificial insemination was performed on three queens, two queens produced 11 viable kittens. To identify the causative mutation, a fibroblast cell line, derived from an affected cat from the original colony, was whole genome sequenced. Visual inspection of the sequence data identified a candidate causal variant as a ~20 kb tandem duplication within LYST, spanning exons 30 through to 38 (NM_001290242.1:c.8347-2422_9548 + 1749dup). PCR genotyping of the produced offspring demonstrated three individuals inherited the mutant allele from the CHS carrier male. This study demonstrated the successful use of cryopreservation and assisted reproduction to maintain and resurrect biomedical models and has defined the variant causing Chediak-Higashi syndrome in the domestic cat.

Original languageEnglish (US)
Article number64
JournalScientific reports
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2020

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Chediak-Higashi Syndrome
Felidae
Reproduction
Cats
Semen
Mutation
Artificial Insemination
Cryopreservation
Exons
Fibroblasts
Alleles
Genome
Cell Line
Polymerase Chain Reaction
Research

ASJC Scopus subject areas

  • General

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Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST. / Buckley, R. M.; Grahn, R. A.; Gandolfi, B.; Herrick, J. R.; Kittleson, M. D.; Bateman, H. L.; Newsom, J.; Swanson, W. F.; Prieur, D. J.; Lyons, L. A.

In: Scientific reports, Vol. 10, No. 1, 64, 01.12.2020.

Research output: Contribution to journalArticle

Buckley, RM, Grahn, RA, Gandolfi, B, Herrick, JR, Kittleson, MD, Bateman, HL, Newsom, J, Swanson, WF, Prieur, DJ & Lyons, LA 2020, 'Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST', Scientific reports, vol. 10, no. 1, 64. https://doi.org/10.1038/s41598-019-56896-9
Buckley, R. M. ; Grahn, R. A. ; Gandolfi, B. ; Herrick, J. R. ; Kittleson, M. D. ; Bateman, H. L. ; Newsom, J. ; Swanson, W. F. ; Prieur, D. J. ; Lyons, L. A. / Assisted reproduction mediated resurrection of a feline model for Chediak-Higashi syndrome caused by a large duplication in LYST. In: Scientific reports. 2020 ; Vol. 10, No. 1.
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abstract = "Chediak-Higashi Syndrome (CHS) is a well-characterized, autosomal recessively inherited lysosomal disease caused by mutations in lysosomal trafficking regulator (LYST). The feline model for CHS was originally maintained for ~20 years. However, the colonies were disbanded and the CHS cat model was lost to the research community before the causative mutation was identified. To resurrect the cat model, semen was collected and cryopreserved from a lone, fertile, CHS carrier male. Using cryopreserved semen, laparoscopic oviductal artificial insemination was performed on three queens, two queens produced 11 viable kittens. To identify the causative mutation, a fibroblast cell line, derived from an affected cat from the original colony, was whole genome sequenced. Visual inspection of the sequence data identified a candidate causal variant as a ~20 kb tandem duplication within LYST, spanning exons 30 through to 38 (NM_001290242.1:c.8347-2422_9548 + 1749dup). PCR genotyping of the produced offspring demonstrated three individuals inherited the mutant allele from the CHS carrier male. This study demonstrated the successful use of cryopreservation and assisted reproduction to maintain and resurrect biomedical models and has defined the variant causing Chediak-Higashi syndrome in the domestic cat.",
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