Abstract
The V79 hamster cell line irs1 is a repair-deficient mutant hypersensitive to radiation and DNA-reactive chemical agents. Somatic cell hybrids were formed by fusing irs1 cells with human lymphocytes and selecting for complementation in medium containing concentrations of mitomycin C (MMC) that are toxic to irs1. Thirty-eight MMC-resistant hybrids showed extensive segregation of human chromosomes, with 35 of them retaining human chromosome 7, as indicated by molecular marker and cytogenetic analyses. Inter-Alu-PCR products from the DNA of hybrids, when used as fluorescence in situ hybridization probe onto normal human metaphases, indicated that one resistant hybrid was monochromosomal for chromosome 7 and that the three resistant hybrids shown to be negative for chromosome 7 markers have retained portions of chromosome 7, with region 7q36 being the smallest common region. MMC-sensitive subclones of a resistant hybrid lost human chromosome 7. Therefore, the gene complementing the repair defect, XRCC2 (X-ray repair cross complementing), is assigned to human chromosome 7q36.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 619-622 |
| Number of pages | 4 |
| Journal | Genomics |
| Volume | 26 |
| Issue number | 3 |
| DOIs | |
| State | Published - Apr 10 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Assignment of the XRCC2 human DNA repair gene to chromosome 7q36 by complementation analysis. / Jones, Nigel J.; Zhao, Ying; Siciliano, Michael J.; Thompson, Larry H.
In: Genomics, Vol. 26, No. 3, 10.04.1995, p. 619-622.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assignment of the XRCC2 human DNA repair gene to chromosome 7q36 by complementation analysis
AU - Jones, Nigel J.
AU - Zhao, Ying
AU - Siciliano, Michael J.
AU - Thompson, Larry H.
PY - 1995/4/10
Y1 - 1995/4/10
N2 - The V79 hamster cell line irs1 is a repair-deficient mutant hypersensitive to radiation and DNA-reactive chemical agents. Somatic cell hybrids were formed by fusing irs1 cells with human lymphocytes and selecting for complementation in medium containing concentrations of mitomycin C (MMC) that are toxic to irs1. Thirty-eight MMC-resistant hybrids showed extensive segregation of human chromosomes, with 35 of them retaining human chromosome 7, as indicated by molecular marker and cytogenetic analyses. Inter-Alu-PCR products from the DNA of hybrids, when used as fluorescence in situ hybridization probe onto normal human metaphases, indicated that one resistant hybrid was monochromosomal for chromosome 7 and that the three resistant hybrids shown to be negative for chromosome 7 markers have retained portions of chromosome 7, with region 7q36 being the smallest common region. MMC-sensitive subclones of a resistant hybrid lost human chromosome 7. Therefore, the gene complementing the repair defect, XRCC2 (X-ray repair cross complementing), is assigned to human chromosome 7q36.
AB - The V79 hamster cell line irs1 is a repair-deficient mutant hypersensitive to radiation and DNA-reactive chemical agents. Somatic cell hybrids were formed by fusing irs1 cells with human lymphocytes and selecting for complementation in medium containing concentrations of mitomycin C (MMC) that are toxic to irs1. Thirty-eight MMC-resistant hybrids showed extensive segregation of human chromosomes, with 35 of them retaining human chromosome 7, as indicated by molecular marker and cytogenetic analyses. Inter-Alu-PCR products from the DNA of hybrids, when used as fluorescence in situ hybridization probe onto normal human metaphases, indicated that one resistant hybrid was monochromosomal for chromosome 7 and that the three resistant hybrids shown to be negative for chromosome 7 markers have retained portions of chromosome 7, with region 7q36 being the smallest common region. MMC-sensitive subclones of a resistant hybrid lost human chromosome 7. Therefore, the gene complementing the repair defect, XRCC2 (X-ray repair cross complementing), is assigned to human chromosome 7q36.
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U2 - 10.1016/0888-7543(95)80187-Q
DO - 10.1016/0888-7543(95)80187-Q
M3 - Article
VL - 26
SP - 619
EP - 622
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 3
ER -