Assignment of proximal histidyl imidazole exchangeable proton nmr resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee

G. N. la Mar; K. Nagai; Thomas Jue; D. L. Budd; K. Gersonde; H. Sick; T. Kagimoto; A. Hayashi; F. Taketa

doi:10.1016/0006-291X(80)90075-3

Assignment of proximal histidyl imidazole exchangeable proton nmr resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee

G. N. la Mar, K. Nagai, Thomas Jue, D. L. Budd, K. Gersonde, H. Sick, T. Kagimoto, A. Hayashi, F. Taketa

Biochemistry and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

The proton nmr spectra of the synthetic valency hybrids, α₂(β⁺CN)₂, (α⁺CN)₂β₂ of hemoglobin A and the natural valency hybrids of the mutant hemoglobins Boston, Iwate and Milwaukee have led to the unambiguous assignment of the two proximal histidyl imidazole exchangeable proton signals at 64 and 76 ppm to individual α and β subunits, respectively. New single non-exchangeable proton resonances detected in the extreme downfield region of the spectra of Hbs Boston and Iwate are tentatively assigned to the coordinated tyrosine of the mutant α chains.

Original language	English (US)
Pages (from-to)	1172-1177
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	96
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(80)90075-3
State	Published - 1980

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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la Mar, G. N., Nagai, K., Jue, T., Budd, D. L., Gersonde, K., Sick, H., Kagimoto, T., Hayashi, A., & Taketa, F. (1980). Assignment of proximal histidyl imidazole exchangeable proton nmr resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee. Biochemical and Biophysical Research Communications, 96(3), 1172-1177. https://doi.org/10.1016/0006-291X(80)90075-3

Assignment of proximal histidyl imidazole exchangeable proton nmr resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee. / la Mar, G. N.; Nagai, K.; Jue, Thomas; Budd, D. L.; Gersonde, K.; Sick, H.; Kagimoto, T.; Hayashi, A.; Taketa, F.

In: Biochemical and Biophysical Research Communications, Vol. 96, No. 3, 1980, p. 1172-1177.

Research output: Contribution to journal › Article › peer-review

la Mar, GN, Nagai, K, Jue, T, Budd, DL, Gersonde, K, Sick, H, Kagimoto, T, Hayashi, A & Taketa, F 1980, 'Assignment of proximal histidyl imidazole exchangeable proton nmr resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee', Biochemical and Biophysical Research Communications, vol. 96, no. 3, pp. 1172-1177. https://doi.org/10.1016/0006-291X(80)90075-3

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title = "Assignment of proximal histidyl imidazole exchangeable proton nmr resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee",

abstract = "The proton nmr spectra of the synthetic valency hybrids, α2(β+CN)2, (α+CN)2β2 of hemoglobin A and the natural valency hybrids of the mutant hemoglobins Boston, Iwate and Milwaukee have led to the unambiguous assignment of the two proximal histidyl imidazole exchangeable proton signals at 64 and 76 ppm to individual α and β subunits, respectively. New single non-exchangeable proton resonances detected in the extreme downfield region of the spectra of Hbs Boston and Iwate are tentatively assigned to the coordinated tyrosine of the mutant α chains.",

author = "{la Mar}, {G. N.} and K. Nagai and Thomas Jue and Budd, {D. L.} and K. Gersonde and H. Sick and T. Kagimoto and A. Hayashi and F. Taketa",

year = "1980",

doi = "10.1016/0006-291X(80)90075-3",

language = "English (US)",

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T1 - Assignment of proximal histidyl imidazole exchangeable proton nmr resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee

AU - la Mar, G. N.

AU - Nagai, K.

AU - Jue, Thomas

AU - Budd, D. L.

AU - Gersonde, K.

AU - Sick, H.

AU - Kagimoto, T.

AU - Hayashi, A.

AU - Taketa, F.

PY - 1980

Y1 - 1980

N2 - The proton nmr spectra of the synthetic valency hybrids, α2(β+CN)2, (α+CN)2β2 of hemoglobin A and the natural valency hybrids of the mutant hemoglobins Boston, Iwate and Milwaukee have led to the unambiguous assignment of the two proximal histidyl imidazole exchangeable proton signals at 64 and 76 ppm to individual α and β subunits, respectively. New single non-exchangeable proton resonances detected in the extreme downfield region of the spectra of Hbs Boston and Iwate are tentatively assigned to the coordinated tyrosine of the mutant α chains.

AB - The proton nmr spectra of the synthetic valency hybrids, α2(β+CN)2, (α+CN)2β2 of hemoglobin A and the natural valency hybrids of the mutant hemoglobins Boston, Iwate and Milwaukee have led to the unambiguous assignment of the two proximal histidyl imidazole exchangeable proton signals at 64 and 76 ppm to individual α and β subunits, respectively. New single non-exchangeable proton resonances detected in the extreme downfield region of the spectra of Hbs Boston and Iwate are tentatively assigned to the coordinated tyrosine of the mutant α chains.

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Assignment of proximal histidyl imidazole exchangeable proton nmr resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee

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