Assignment of a human DNA-repair gene associated with sister-chromatid exchange to chromosome 19

M. J. Siciliano, A. V. Carrano, L. H. Thompson

Research output: Contribution to journalArticle

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Abstract

The Chinese hamster ovary (CHO) cell mutant, EM9, is defective in rejoining strand breaks, hypersensitive to chlorodeoxyuridine (CldUrd), and has a high frequency of sister-chromatid exchange (SCE). Somatic cell hybrids constructed from fusion of EM9 cells with normal human lymphocytes and fibroblasts, and selected in CldUrd, extensively segregate human chromosomes but preferentially retain markers of human chromosome 19. The SCE frequency in the hybrid clones is low as in normal CHO cells, but in CldUrd-sensitive subclones, which lose the human chromosome 19 markers, SCE frequencies return to mutant levels. We therefore assign a human gene designated repair complementing defective repair in Chinese-hamster (RCC) to chromosome 19. Since this is the second (of two) human genes complementing repair-deficiency mutations in CHO cells assigned to the 19, the assignment and organization of DNA-repair genes is discussed in the light of hemizygosity in CHO cells and the evolutionary conservation of mammalian linkage groups.

Original languageEnglish (US)
Pages (from-to)303-308
Number of pages6
JournalMutation Research Letters
Volume174
Issue number4
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 19
Sister Chromatid Exchange
Chromosomes
Cricetulus
DNA Repair
Repair
Genes
Ovary
Human Chromosomes
DNA
Lymphocytes
Fibroblasts
Cell Fusion
Hybrid Cells
Conservation
Genetic Markers
Fusion reactions
Cells
Clone Cells
Mutation

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

Cite this

Assignment of a human DNA-repair gene associated with sister-chromatid exchange to chromosome 19. / Siciliano, M. J.; Carrano, A. V.; Thompson, L. H.

In: Mutation Research Letters, Vol. 174, No. 4, 1986, p. 303-308.

Research output: Contribution to journalArticle

Siciliano, M. J. ; Carrano, A. V. ; Thompson, L. H. / Assignment of a human DNA-repair gene associated with sister-chromatid exchange to chromosome 19. In: Mutation Research Letters. 1986 ; Vol. 174, No. 4. pp. 303-308.
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