Assessment of nutritional status in rhesus monkeys: Comparison of dual-energy X-ray absorptiometry and stable isotope dilution

Stéphane Blanc, Ricki Colman, Joseph Kemnitz, Richard Weindruch, Scott Baum, Jon J Ramsey, Dale Schoeller

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.

Original languageEnglish (US)
Pages (from-to)130-138
Number of pages9
JournalJournal of Medical Primatology
Volume34
Issue number3
DOIs
StatePublished - Jun 2005

Fingerprint

dual-energy X-ray absorptiometry
isotope dilution technique
Photon Absorptiometry
Macaca mulatta
Nutritional Status
Isotopes
stable isotopes
nutritional status
Fats
lipids
Body Composition
body composition
energy
Haplorhini
monkeys
Software
animals
methodology

Keywords

  • Absorptiometry
  • Body composition
  • Stable isotopes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Assessment of nutritional status in rhesus monkeys : Comparison of dual-energy X-ray absorptiometry and stable isotope dilution. / Blanc, Stéphane; Colman, Ricki; Kemnitz, Joseph; Weindruch, Richard; Baum, Scott; Ramsey, Jon J; Schoeller, Dale.

In: Journal of Medical Primatology, Vol. 34, No. 3, 06.2005, p. 130-138.

Research output: Contribution to journalArticle

Blanc, Stéphane ; Colman, Ricki ; Kemnitz, Joseph ; Weindruch, Richard ; Baum, Scott ; Ramsey, Jon J ; Schoeller, Dale. / Assessment of nutritional status in rhesus monkeys : Comparison of dual-energy X-ray absorptiometry and stable isotope dilution. In: Journal of Medical Primatology. 2005 ; Vol. 34, No. 3. pp. 130-138.
@article{d75e143001cb47f4bb5dc3e44babb6d3,
title = "Assessment of nutritional status in rhesus monkeys: Comparison of dual-energy X-ray absorptiometry and stable isotope dilution",
abstract = "Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51{\%} were in the energy restricted group and 38{\%} were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5{\%}, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6{\%}, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5{\%}, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20{\%}, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.",
keywords = "Absorptiometry, Body composition, Stable isotopes",
author = "St{\'e}phane Blanc and Ricki Colman and Joseph Kemnitz and Richard Weindruch and Scott Baum and Ramsey, {Jon J} and Dale Schoeller",
year = "2005",
month = "6",
doi = "10.1111/j.1600-0684.2005.00106.x",
language = "English (US)",
volume = "34",
pages = "130--138",
journal = "Journal of Medical Primatology",
issn = "0047-2565",
publisher = "Blackwell Munksgaard",
number = "3",

}

TY - JOUR

T1 - Assessment of nutritional status in rhesus monkeys

T2 - Comparison of dual-energy X-ray absorptiometry and stable isotope dilution

AU - Blanc, Stéphane

AU - Colman, Ricki

AU - Kemnitz, Joseph

AU - Weindruch, Richard

AU - Baum, Scott

AU - Ramsey, Jon J

AU - Schoeller, Dale

PY - 2005/6

Y1 - 2005/6

N2 - Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.

AB - Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.

KW - Absorptiometry

KW - Body composition

KW - Stable isotopes

UR - http://www.scopus.com/inward/record.url?scp=19044362501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19044362501&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0684.2005.00106.x

DO - 10.1111/j.1600-0684.2005.00106.x

M3 - Article

C2 - 15860121

AN - SCOPUS:19044362501

VL - 34

SP - 130

EP - 138

JO - Journal of Medical Primatology

JF - Journal of Medical Primatology

SN - 0047-2565

IS - 3

ER -