TY - JOUR
T1 - Assessment of nutritional status in rhesus monkeys
T2 - Comparison of dual-energy X-ray absorptiometry and stable isotope dilution
AU - Blanc, Stéphane
AU - Colman, Ricki
AU - Kemnitz, Joseph
AU - Weindruch, Richard
AU - Baum, Scott
AU - Ramsey, Jon J
AU - Schoeller, Dale
PY - 2005/6
Y1 - 2005/6
N2 - Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.
AB - Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.
KW - Absorptiometry
KW - Body composition
KW - Stable isotopes
UR - http://www.scopus.com/inward/record.url?scp=19044362501&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19044362501&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0684.2005.00106.x
DO - 10.1111/j.1600-0684.2005.00106.x
M3 - Article
C2 - 15860121
AN - SCOPUS:19044362501
VL - 34
SP - 130
EP - 138
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
SN - 0047-2565
IS - 3
ER -