Abstract
Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 130-138 |
| Number of pages | 9 |
| Journal | Journal of Medical Primatology |
| Volume | 34 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2005 |
Fingerprint
Keywords
- Absorptiometry
- Body composition
- Stable isotopes
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
Assessment of nutritional status in rhesus monkeys : Comparison of dual-energy X-ray absorptiometry and stable isotope dilution. / Blanc, Stéphane; Colman, Ricki; Kemnitz, Joseph; Weindruch, Richard; Baum, Scott; Ramsey, Jon J; Schoeller, Dale.
In: Journal of Medical Primatology, Vol. 34, No. 3, 06.2005, p. 130-138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assessment of nutritional status in rhesus monkeys
T2 - Comparison of dual-energy X-ray absorptiometry and stable isotope dilution
AU - Blanc, Stéphane
AU - Colman, Ricki
AU - Kemnitz, Joseph
AU - Weindruch, Richard
AU - Baum, Scott
AU - Ramsey, Jon J
AU - Schoeller, Dale
PY - 2005/6
Y1 - 2005/6
N2 - Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.
AB - Body composition estimates from dual-energy X-ray absorptiometry and stable isotope dilution (2H and 18O) were compared in 61 rhesus monkeys (Macaca mulatta) from the ongoing long-term energy restriction study at the University of Wisconsin. Their average age was 18.9 ± 2.5 y/o. Of the animals, 51% were in the energy restricted group and 38% were females. Although the correlation between methods was highly significant for fat mass (R2 = 0.97, SEE = 0.25 kg or 7.5%, P < 0.0001) and fat-free mass (R2 = 0.98, SEE = 0.29 kg or 3.6%, P < 0.0001), we observed that dual-energy X-ray absorptiometry underestimated fat mass by 0.67 ± 0.26 kg (7.5%, P < 0.0001) and overestimated fat-free mass by 0.57 ± 0.29 kg (20%, P < 0.0001) when compared with isotope dilution. Taken together with data from the literature, the present results emphasize the usefulness of dual-energy X-ray absorptiometry to derive body composition and thus nutritional status in monkeys, but demonstrate the importance of validation experiments for a given DXA model and software.
KW - Absorptiometry
KW - Body composition
KW - Stable isotopes
UR - http://www.scopus.com/inward/record.url?scp=19044362501&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19044362501&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0684.2005.00106.x
DO - 10.1111/j.1600-0684.2005.00106.x
M3 - Article
C2 - 15860121
AN - SCOPUS:19044362501
VL - 34
SP - 130
EP - 138
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
SN - 0047-2565
IS - 3
ER -