Assessment of IgE response and cytokine gene expression in pulmonary efferent lymph collected after ovalbumin inhalation during experimental infection of calves with bovine respiratory syncytial virus

Laurel J Gershwin, Mark L Anderson, Chunbo Wang, Londa J. Berghaus, Thomas P. Kenny, Robert A. Gunther

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Objective-To assess IgE response and cytokine gene expressions in pulmonary lymph collected from bovine respiratory syncytial virus (BRSV)-infected calves after ovalbumin inhalation. Animals-Thirteen 7- to 8-week-old calves. Procedures-The efferent lymphatic duct of the caudal mediastinal lymph node of each calf was cannulated 3 or 4 days before experiment commencement. Calves were inoculated (day 0) with BRSV (n = 7) or BRSV-free tissue culture medium (mock exposure; 6) via aerosolization and exposed to aerosolized ovalbumin on days 1 through 6 and day 15. An efferent lymph sample was collected daily from each calf on days -1 through 16; CD4+ and CD8+ T lymphocyte subsets in lymph samples were enumerated with a fluorescenceactivated cell scanner. Expressions of several cytokines by efferent lymphocytes and lymph ovalbumin-specific IgE concentration were measured. Each calf was euthanized on day 16 and then necropsied for evaluation of lungs. Results-Mean fold increase in ovalbumin-specific IgE concentration was greater in BRSVinfected calves than in mock-infected calves. At various time points from days 4 through 10, percentages of T lymphocyte subsets and CD4+:CD8+ T lymphocyte ratios differed between BRSV-infected calves and day -1 values or from values in mock-infected calves. On days 3 through 5, IL-4 and IL-13 gene expressions in BRSV-infected calves were increased, compared with expressions in mock-infected calves. Lung lesions were consistent with antigen exposure. Conclusions and Clinical Relevance-In response to the inhalation of aerosolized ovalbumin, BRSV infection in calves appeared to facilitate induction of a T helper 2 cell response and ovalbumin-specific IgE production. (Am J Vet Res 2011;72:134-145).

Original languageEnglish (US)
Pages (from-to)134-145
Number of pages12
JournalAmerican Journal of Veterinary Research
Volume72
Issue number1
DOIs
StatePublished - Jan 2011

ASJC Scopus subject areas

  • veterinary(all)

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