Assessment of growth factor treatment on fibrochondrocyte and chondrocyte co-cultures for TMJ fibrocartilage engineering

Kerem N. Kalpakci, Eric J. Kim, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Treatments for patients suffering from severe temporomandibular joint (TMJ) dysfunction are limited, motivating the development of strategies for tissue regeneration. In this study, co-cultures of fibrochondrocytes (FCs) and articular chondrocytes (ACs) were seeded in agarose wells, and supplemented with growth factors, to engineer tissue with biomechanical properties and extracellular matrix composition similar to native TMJ fibrocartilage. In the first phase, growth factors were applied alone and in combination, in the presence or absence of serum, while in the second phase, the best overall treatment was applied at intermittent dosing. Continuous treatment of AC/FC co-cultures with TGF-β1 in serum-free medium resulted in constructs with glycosaminoglycan/wet weight ratios (12.2%), instantaneous compressive moduli (790 kPa), relaxed compressive moduli (120 kPa) and Young's moduli (1.87 MPa) that overlap with native TMJ disc values. Among co-culture groups, TGF-β1 treatment increased collagen deposition ∼20%, compressive stiffness ∼130% and Young's modulus ∼170% relative to controls without growth factor. Serum supplementation, though generally detrimental to functional properties, was identified as a powerful mediator of FC construct morphology. Finally, both intermittent and continuous TGF-β1 treatment showed positive effects, though continuous treatment resulted in greater enhancement of construct functional properties. This work proposes a strategy for regeneration of TMJ fibrocartilage and its future application will be realized through translation of these findings to clinically viable cell sources.

Original languageEnglish (US)
Pages (from-to)1710-1718
Number of pages9
JournalActa Biomaterialia
Volume7
Issue number4
DOIs
StatePublished - Apr 2011

Fingerprint

Fibrocartilage
Temporomandibular Joint
Chondrocytes
Coculture Techniques
Intercellular Signaling Peptides and Proteins
Elastic Modulus
Elastic moduli
Tissue regeneration
Regeneration
Serum-Free Culture Media
Therapeutics
Joints
Glycosaminoglycans
Temporomandibular Joint Disc
Collagen
Sepharose
Stiffness
Tissue
Serum
Engineers

Keywords

  • Fibrocartilage
  • Growth factors
  • Scaffoldless
  • Tissue engineering
  • TMJ

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Assessment of growth factor treatment on fibrochondrocyte and chondrocyte co-cultures for TMJ fibrocartilage engineering. / Kalpakci, Kerem N.; Kim, Eric J.; Athanasiou, Kyriacos A.

In: Acta Biomaterialia, Vol. 7, No. 4, 04.2011, p. 1710-1718.

Research output: Contribution to journalArticle

Kalpakci, Kerem N. ; Kim, Eric J. ; Athanasiou, Kyriacos A. / Assessment of growth factor treatment on fibrochondrocyte and chondrocyte co-cultures for TMJ fibrocartilage engineering. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 4. pp. 1710-1718.
@article{ee74568aa39a4688b4aede4e8d105745,
title = "Assessment of growth factor treatment on fibrochondrocyte and chondrocyte co-cultures for TMJ fibrocartilage engineering",
abstract = "Treatments for patients suffering from severe temporomandibular joint (TMJ) dysfunction are limited, motivating the development of strategies for tissue regeneration. In this study, co-cultures of fibrochondrocytes (FCs) and articular chondrocytes (ACs) were seeded in agarose wells, and supplemented with growth factors, to engineer tissue with biomechanical properties and extracellular matrix composition similar to native TMJ fibrocartilage. In the first phase, growth factors were applied alone and in combination, in the presence or absence of serum, while in the second phase, the best overall treatment was applied at intermittent dosing. Continuous treatment of AC/FC co-cultures with TGF-β1 in serum-free medium resulted in constructs with glycosaminoglycan/wet weight ratios (12.2{\%}), instantaneous compressive moduli (790 kPa), relaxed compressive moduli (120 kPa) and Young's moduli (1.87 MPa) that overlap with native TMJ disc values. Among co-culture groups, TGF-β1 treatment increased collagen deposition ∼20{\%}, compressive stiffness ∼130{\%} and Young's modulus ∼170{\%} relative to controls without growth factor. Serum supplementation, though generally detrimental to functional properties, was identified as a powerful mediator of FC construct morphology. Finally, both intermittent and continuous TGF-β1 treatment showed positive effects, though continuous treatment resulted in greater enhancement of construct functional properties. This work proposes a strategy for regeneration of TMJ fibrocartilage and its future application will be realized through translation of these findings to clinically viable cell sources.",
keywords = "Fibrocartilage, Growth factors, Scaffoldless, Tissue engineering, TMJ",
author = "Kalpakci, {Kerem N.} and Kim, {Eric J.} and Athanasiou, {Kyriacos A.}",
year = "2011",
month = "4",
doi = "10.1016/j.actbio.2010.12.015",
language = "English (US)",
volume = "7",
pages = "1710--1718",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - Assessment of growth factor treatment on fibrochondrocyte and chondrocyte co-cultures for TMJ fibrocartilage engineering

AU - Kalpakci, Kerem N.

AU - Kim, Eric J.

AU - Athanasiou, Kyriacos A.

PY - 2011/4

Y1 - 2011/4

N2 - Treatments for patients suffering from severe temporomandibular joint (TMJ) dysfunction are limited, motivating the development of strategies for tissue regeneration. In this study, co-cultures of fibrochondrocytes (FCs) and articular chondrocytes (ACs) were seeded in agarose wells, and supplemented with growth factors, to engineer tissue with biomechanical properties and extracellular matrix composition similar to native TMJ fibrocartilage. In the first phase, growth factors were applied alone and in combination, in the presence or absence of serum, while in the second phase, the best overall treatment was applied at intermittent dosing. Continuous treatment of AC/FC co-cultures with TGF-β1 in serum-free medium resulted in constructs with glycosaminoglycan/wet weight ratios (12.2%), instantaneous compressive moduli (790 kPa), relaxed compressive moduli (120 kPa) and Young's moduli (1.87 MPa) that overlap with native TMJ disc values. Among co-culture groups, TGF-β1 treatment increased collagen deposition ∼20%, compressive stiffness ∼130% and Young's modulus ∼170% relative to controls without growth factor. Serum supplementation, though generally detrimental to functional properties, was identified as a powerful mediator of FC construct morphology. Finally, both intermittent and continuous TGF-β1 treatment showed positive effects, though continuous treatment resulted in greater enhancement of construct functional properties. This work proposes a strategy for regeneration of TMJ fibrocartilage and its future application will be realized through translation of these findings to clinically viable cell sources.

AB - Treatments for patients suffering from severe temporomandibular joint (TMJ) dysfunction are limited, motivating the development of strategies for tissue regeneration. In this study, co-cultures of fibrochondrocytes (FCs) and articular chondrocytes (ACs) were seeded in agarose wells, and supplemented with growth factors, to engineer tissue with biomechanical properties and extracellular matrix composition similar to native TMJ fibrocartilage. In the first phase, growth factors were applied alone and in combination, in the presence or absence of serum, while in the second phase, the best overall treatment was applied at intermittent dosing. Continuous treatment of AC/FC co-cultures with TGF-β1 in serum-free medium resulted in constructs with glycosaminoglycan/wet weight ratios (12.2%), instantaneous compressive moduli (790 kPa), relaxed compressive moduli (120 kPa) and Young's moduli (1.87 MPa) that overlap with native TMJ disc values. Among co-culture groups, TGF-β1 treatment increased collagen deposition ∼20%, compressive stiffness ∼130% and Young's modulus ∼170% relative to controls without growth factor. Serum supplementation, though generally detrimental to functional properties, was identified as a powerful mediator of FC construct morphology. Finally, both intermittent and continuous TGF-β1 treatment showed positive effects, though continuous treatment resulted in greater enhancement of construct functional properties. This work proposes a strategy for regeneration of TMJ fibrocartilage and its future application will be realized through translation of these findings to clinically viable cell sources.

KW - Fibrocartilage

KW - Growth factors

KW - Scaffoldless

KW - Tissue engineering

KW - TMJ

UR - http://www.scopus.com/inward/record.url?scp=79952195385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952195385&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2010.12.015

DO - 10.1016/j.actbio.2010.12.015

M3 - Article

C2 - 21185408

AN - SCOPUS:79952195385

VL - 7

SP - 1710

EP - 1718

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 4

ER -