Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury

Benxiu Ji; Lauren C. Case; Kai Liu; Zhaohui Shao; Xinhua Lee; Zhongshu Yang; Joy Wang; Tim Tian; Svetlana Shulga-Morskaya; Martin Scott; Zhigang He; Jane K. Relton; Sha Mi

doi:10.1016/j.mcn.2008.07.004

Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury

Benxiu Ji, Lauren C. Case, Kai Liu, Zhaohui Shao, Xinhua Lee, Zhongshu Yang, Joy Wang, Tim Tian, Svetlana Shulga-Morskaya, Martin Scott, Zhigang He, Jane K. Relton, Sha Mi

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Oligodendrocyte-myelin glycoprotein (OMgp) is a myelin component that has been shown in vitro to inhibit neurite outgrowth by binding to the Nogo-66 receptor (NgR1)/Lingo-1/Taj (TROY)/p75 receptor complex to activate the RhoA pathway. To investigate the effects of OMgp on axon regeneration in vivo, OMgp^-/- mice on a mixed 129/Sv/C57BL/6 (129BL6) or a C57BL/6 (BL6) genetic background were tested in two spinal cord injury (SCI) models - a severe complete transection or a milder dorsal hemisection. OMgp^-/- mice on the mixed 129BL6 genetic background showed greater functional improvement compared to OMgp^+/+ littermates, with increased numbers of cholera toxin B-labeled ascending sensory axons and 5-HT⁺ descending axons and less RhoA activation after spinal cord injury. Myelin isolated from OMgp^-/- mice (129BL6) was significantly less inhibitory to neurite outgrowth than wild-type (wt) myelin in vitro. However, OMgp^-/- mice on a BL/6 genetic background showed neither statistically significant functional recovery nor axonal sprouting following dorsal hemisection.

Original language	English (US)
Pages (from-to)	258-267
Number of pages	10
Journal	Molecular and Cellular Neuroscience
Volume	39
Issue number	2
DOIs	https://doi.org/10.1016/j.mcn.2008.07.004
State	Published - Oct 2008
Externally published	Yes

Keywords

Axonal regeneration
MAG
Myelin
Nogo
OMgp
Spinal cord injury

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience
Developmental Neuroscience

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Ji, B., Case, L. C., Liu, K., Shao, Z., Lee, X., Yang, Z., Wang, J., Tian, T., Shulga-Morskaya, S., Scott, M., He, Z., Relton, J. K., & Mi, S. (2008). Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury. Molecular and Cellular Neuroscience, 39(2), 258-267. https://doi.org/10.1016/j.mcn.2008.07.004

Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury. / Ji, Benxiu; Case, Lauren C.; Liu, Kai; Shao, Zhaohui; Lee, Xinhua; Yang, Zhongshu; Wang, Joy; Tian, Tim; Shulga-Morskaya, Svetlana; Scott, Martin; He, Zhigang; Relton, Jane K.; Mi, Sha.

In: Molecular and Cellular Neuroscience, Vol. 39, No. 2, 10.2008, p. 258-267.

Research output: Contribution to journal › Article › peer-review

Ji, B, Case, LC, Liu, K, Shao, Z, Lee, X, Yang, Z, Wang, J, Tian, T, Shulga-Morskaya, S, Scott, M, He, Z, Relton, JK & Mi, S 2008, 'Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury', Molecular and Cellular Neuroscience, vol. 39, no. 2, pp. 258-267. https://doi.org/10.1016/j.mcn.2008.07.004

Ji, Benxiu ; Case, Lauren C. ; Liu, Kai ; Shao, Zhaohui ; Lee, Xinhua ; Yang, Zhongshu ; Wang, Joy ; Tian, Tim ; Shulga-Morskaya, Svetlana ; Scott, Martin ; He, Zhigang ; Relton, Jane K. ; Mi, Sha. / Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury. In: Molecular and Cellular Neuroscience. 2008 ; Vol. 39, No. 2. pp. 258-267.

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abstract = "Oligodendrocyte-myelin glycoprotein (OMgp) is a myelin component that has been shown in vitro to inhibit neurite outgrowth by binding to the Nogo-66 receptor (NgR1)/Lingo-1/Taj (TROY)/p75 receptor complex to activate the RhoA pathway. To investigate the effects of OMgp on axon regeneration in vivo, OMgp-/- mice on a mixed 129/Sv/C57BL/6 (129BL6) or a C57BL/6 (BL6) genetic background were tested in two spinal cord injury (SCI) models - a severe complete transection or a milder dorsal hemisection. OMgp-/- mice on the mixed 129BL6 genetic background showed greater functional improvement compared to OMgp+/+ littermates, with increased numbers of cholera toxin B-labeled ascending sensory axons and 5-HT+ descending axons and less RhoA activation after spinal cord injury. Myelin isolated from OMgp-/- mice (129BL6) was significantly less inhibitory to neurite outgrowth than wild-type (wt) myelin in vitro. However, OMgp-/- mice on a BL/6 genetic background showed neither statistically significant functional recovery nor axonal sprouting following dorsal hemisection.",

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Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury

Abstract

Keywords

ASJC Scopus subject areas

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