Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury

Benxiu Ji, Lauren C. Case, Kai Liu, Zhaohui Shao, Xinhua Lee, Zhongshu Yang, Joy Wang, Tim Tian, Svetlana Shulga-Morskaya, Martin Scott, Zhigang He, Jane K. Relton, Sha Mi

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Oligodendrocyte-myelin glycoprotein (OMgp) is a myelin component that has been shown in vitro to inhibit neurite outgrowth by binding to the Nogo-66 receptor (NgR1)/Lingo-1/Taj (TROY)/p75 receptor complex to activate the RhoA pathway. To investigate the effects of OMgp on axon regeneration in vivo, OMgp-/- mice on a mixed 129/Sv/C57BL/6 (129BL6) or a C57BL/6 (BL6) genetic background were tested in two spinal cord injury (SCI) models - a severe complete transection or a milder dorsal hemisection. OMgp-/- mice on the mixed 129BL6 genetic background showed greater functional improvement compared to OMgp+/+ littermates, with increased numbers of cholera toxin B-labeled ascending sensory axons and 5-HT+ descending axons and less RhoA activation after spinal cord injury. Myelin isolated from OMgp-/- mice (129BL6) was significantly less inhibitory to neurite outgrowth than wild-type (wt) myelin in vitro. However, OMgp-/- mice on a BL/6 genetic background showed neither statistically significant functional recovery nor axonal sprouting following dorsal hemisection.

Original languageEnglish (US)
Pages (from-to)258-267
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume39
Issue number2
DOIs
StatePublished - Oct 2008
Externally publishedYes

Keywords

  • Axonal regeneration
  • MAG
  • Myelin
  • Nogo
  • OMgp
  • Spinal cord injury

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

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  • Cite this

    Ji, B., Case, L. C., Liu, K., Shao, Z., Lee, X., Yang, Z., Wang, J., Tian, T., Shulga-Morskaya, S., Scott, M., He, Z., Relton, J. K., & Mi, S. (2008). Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury. Molecular and Cellular Neuroscience, 39(2), 258-267. https://doi.org/10.1016/j.mcn.2008.07.004