Abstract
Oligodendrocyte-myelin glycoprotein (OMgp) is a myelin component that has been shown in vitro to inhibit neurite outgrowth by binding to the Nogo-66 receptor (NgR1)/Lingo-1/Taj (TROY)/p75 receptor complex to activate the RhoA pathway. To investigate the effects of OMgp on axon regeneration in vivo, OMgp-/- mice on a mixed 129/Sv/C57BL/6 (129BL6) or a C57BL/6 (BL6) genetic background were tested in two spinal cord injury (SCI) models - a severe complete transection or a milder dorsal hemisection. OMgp-/- mice on the mixed 129BL6 genetic background showed greater functional improvement compared to OMgp+/+ littermates, with increased numbers of cholera toxin B-labeled ascending sensory axons and 5-HT+ descending axons and less RhoA activation after spinal cord injury. Myelin isolated from OMgp-/- mice (129BL6) was significantly less inhibitory to neurite outgrowth than wild-type (wt) myelin in vitro. However, OMgp-/- mice on a BL/6 genetic background showed neither statistically significant functional recovery nor axonal sprouting following dorsal hemisection.
Original language | English (US) |
---|---|
Pages (from-to) | 258-267 |
Number of pages | 10 |
Journal | Molecular and Cellular Neuroscience |
Volume | 39 |
Issue number | 2 |
DOIs | |
State | Published - Oct 2008 |
Externally published | Yes |
Keywords
- Axonal regeneration
- MAG
- Myelin
- Nogo
- OMgp
- Spinal cord injury
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
- Developmental Neuroscience