Assessment of cell binding to hyaluronic acid in a solid-phase assay

Margaret M. Lee Guo, James Hildreth

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Adhesion to hyaluronate by tumor cells has been shown to correlate with disease progression in various types of neoplasms. In order to study cell binding to hyaluronate, a novel assay was developed with hyaluronate covalently tethered to polystyrene plates in 96-well format. Hyaluronic acid is covalently linked to amine groups on the surface of these plates by 1- ethyl-3(3-dimethylaminopropyl) carbodiimide. The amide linkage is stable to repeated washings, and cell binding to this derivatized surface is highly reproducible. Use of plates coated with hyaluronate by this new method provides an ideal in vitro model system for assaying cell binding.

Original languageEnglish (US)
Pages (from-to)216-220
Number of pages5
JournalAnalytical Biochemistry
Volume233
Issue number2
DOIs
StatePublished - Jan 15 1996
Externally publishedYes

Fingerprint

Hyaluronic Acid
Assays
Ethyldimethylaminopropyl Carbodiimide
Polystyrenes
Washing
Amides
Amines
Tumors
Adhesion
Cells
Disease Progression
Neoplasms

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Assessment of cell binding to hyaluronic acid in a solid-phase assay. / Lee Guo, Margaret M.; Hildreth, James.

In: Analytical Biochemistry, Vol. 233, No. 2, 15.01.1996, p. 216-220.

Research output: Contribution to journalArticle

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