Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors

Isabel Pérez-Otaño, Christine T. Schulteis, Anis Contractor, Stuart A. Lipton, James Trimmer, Nikolaus J. Sucher, Stephen F. Heinemann

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

Functional NMDA receptors are heteromultimeric complexes of the NR1 subunit in combination with at least one of the four NR2 subunits (A-D). Coexpression of NR3A, an additional subunit of the NMDA receptor family, modifies NMDA-mediated responses. It is unclear whether NR3A interacts directly with NR1 and/or NR2 subunits and how such association might regulate the intracellular trafficking and membrane expression of NR3A. Here we show that NR3A coassembles with NR1-1a and NR2A to form a receptor complex with distinct single-channel properties and a reduced relative calcium permeability. NR3A associates independently with both NR1-1a and NR2A in the endoplasmic reticulum, but only heteromeric complexes containing the NR1-1a NMDA receptor subunit are targeted to the plasma membrane, Homomeric NR3A complexes or complexes composed of NR2A and NR3A were not detected on the cell surface and are retained in the endoplasmic reticulum. Coexpression of NR1-1a facilitates the surface expression of NR3A-containing receptors, reduces the accumulation of NR3A subunits in the endoplasmic reticulum, and induces the appearance of intracellular clusters where both subunits are colocalized. Our data demonstrate a role for subunit oligomerization and specifically assembly with the NR1 subunit in the trafficking and plasma membrane targeting of the receptor complex.

Original languageEnglish (US)
Pages (from-to)1228-1237
Number of pages10
JournalJournal of Neuroscience
Volume21
Issue number4
StatePublished - Feb 15 2001
Externally publishedYes

Fingerprint

Endoplasmic Reticulum
N-Methyl-D-Aspartate Receptors
Cell Membrane
Intracellular Membranes
N-Methylaspartate
Permeability
Calcium
NR3A NMDA receptor
NR1 NMDA receptor

Keywords

  • Assembly
  • Calcium permeability
  • Glutamate
  • NMDA receptor
  • NR3A
  • Single channel
  • Surface expression

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pérez-Otaño, I., Schulteis, C. T., Contractor, A., Lipton, S. A., Trimmer, J., Sucher, N. J., & Heinemann, S. F. (2001). Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors. Journal of Neuroscience, 21(4), 1228-1237.

Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors. / Pérez-Otaño, Isabel; Schulteis, Christine T.; Contractor, Anis; Lipton, Stuart A.; Trimmer, James; Sucher, Nikolaus J.; Heinemann, Stephen F.

In: Journal of Neuroscience, Vol. 21, No. 4, 15.02.2001, p. 1228-1237.

Research output: Contribution to journalArticle

Pérez-Otaño, I, Schulteis, CT, Contractor, A, Lipton, SA, Trimmer, J, Sucher, NJ & Heinemann, SF 2001, 'Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors', Journal of Neuroscience, vol. 21, no. 4, pp. 1228-1237.
Pérez-Otaño I, Schulteis CT, Contractor A, Lipton SA, Trimmer J, Sucher NJ et al. Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors. Journal of Neuroscience. 2001 Feb 15;21(4):1228-1237.
Pérez-Otaño, Isabel ; Schulteis, Christine T. ; Contractor, Anis ; Lipton, Stuart A. ; Trimmer, James ; Sucher, Nikolaus J. ; Heinemann, Stephen F. / Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 4. pp. 1228-1237.
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