Abstract
Rearrangements of the IgH locus with J(H) joined to reading frame 2 of D(H) are greatly underrepresented in B cells. These rearrangements encode the truncated heavy chain Dμ. In pre-B cells, we found Dμ protein expressed on the cell surface and assembled into a complex with surrogate light chains, Igα, and Igβ. Cross-linking of either μm- or Dμm-containing pre-B cell receptors triggered signal transduction reactions. In contrast, when expressed in mature B cell lines, Dμ was not detected on the cell surface and did not efficiently bind κ immunoglobulin light chains, but did associate with Igα and Igβ. These results characterize the interactions of Dμ chain with other components of the B cell antigen receptor complex and suggest possible mechanisms by which Dμ expression may interfere with B cell development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-158 |
| Number of pages | 14 |
| Journal | Immunity |
| Volume | 4 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
- Immunology
Cite this
Assembly of the truncated immunoglobulin heavy chain Dμ into antigen receptor-like complexes in pre-B cells but not in B cells. / Horne, Mary C; Roth, Patricia E.; DeFranco, Anthony L.
In: Immunity, Vol. 4, No. 2, 02.1996, p. 145-158.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assembly of the truncated immunoglobulin heavy chain Dμ into antigen receptor-like complexes in pre-B cells but not in B cells
AU - Horne, Mary C
AU - Roth, Patricia E.
AU - DeFranco, Anthony L.
PY - 1996/2
Y1 - 1996/2
N2 - Rearrangements of the IgH locus with J(H) joined to reading frame 2 of D(H) are greatly underrepresented in B cells. These rearrangements encode the truncated heavy chain Dμ. In pre-B cells, we found Dμ protein expressed on the cell surface and assembled into a complex with surrogate light chains, Igα, and Igβ. Cross-linking of either μm- or Dμm-containing pre-B cell receptors triggered signal transduction reactions. In contrast, when expressed in mature B cell lines, Dμ was not detected on the cell surface and did not efficiently bind κ immunoglobulin light chains, but did associate with Igα and Igβ. These results characterize the interactions of Dμ chain with other components of the B cell antigen receptor complex and suggest possible mechanisms by which Dμ expression may interfere with B cell development.
AB - Rearrangements of the IgH locus with J(H) joined to reading frame 2 of D(H) are greatly underrepresented in B cells. These rearrangements encode the truncated heavy chain Dμ. In pre-B cells, we found Dμ protein expressed on the cell surface and assembled into a complex with surrogate light chains, Igα, and Igβ. Cross-linking of either μm- or Dμm-containing pre-B cell receptors triggered signal transduction reactions. In contrast, when expressed in mature B cell lines, Dμ was not detected on the cell surface and did not efficiently bind κ immunoglobulin light chains, but did associate with Igα and Igβ. These results characterize the interactions of Dμ chain with other components of the B cell antigen receptor complex and suggest possible mechanisms by which Dμ expression may interfere with B cell development.
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U2 - 10.1016/S1074-7613(00)80679-2
DO - 10.1016/S1074-7613(00)80679-2
M3 - Article
VL - 4
SP - 145
EP - 158
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 2
ER -