Assembly of oriented virus arrays by chemo-selective ligation methods and nanolithography techniques

Julio A. Camarero; Chin Li Cheung; Tianwei Lin; John E. Johnson; Brandon L. Weeks; Aleksandr Noy; Jim J. De Yoreo

Assembly of oriented virus arrays by chemo-selective ligation methods and nanolithography techniques

Julio A. Camarero, Chin Li Cheung, Tianwei Lin, John E. Johnson, Brandon L. Weeks, Aleksandr Noy, Jim J. De Yoreo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The present work describes our ongoing efforts towards the creation of nano-scaled ordered arrays of protein/virus covalently attached to site-specific chemical linkers patterned by different nanolithograpy techniques. We will present a new and efficient solid-phase approach for the synthesis of chemically modified long alkyl-thiols. These compounds can be used to introduce chemoselective reacting groups onto gold and silicon-based surfaces. Furthermore, these modified thiols have been used to create nanometric patterns by using different nanolithography techniques. We will show that this patterns can react chemoselectively with proteins and/or virus which have been chemically or recombinantly modified to contain complementary chemical groups at specific positions thus resulting in the oriented attachment of the protein or virus to the surface.

Original language	English (US)
Title of host publication	2003 Nanotechnology Conference and Trade Show - Nanotech 2003
Editors	M. Laudon, B. Romanowicz
Pages	78-80
Number of pages	3
Volume	1
State	Published - 2003
Externally published	Yes
Event	2003 Nanotechnology Conference and Trade Show - Nanotech 2003 - San Francisco, CA, United States Duration: Feb 23 2003 → Feb 27 2003

Other

Other	2003 Nanotechnology Conference and Trade Show - Nanotech 2003
Country	United States
City	San Francisco, CA
Period	2/23/03 → 2/27/03

Keywords

Chemoselective Attachment
Cow Pea Mosaic Virus (CPMV)
Dip Pen Nanolithography
Protein Arrays

ASJC Scopus subject areas

Engineering(all)

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Assembly of oriented virus arrays by chemo-selective ligation methods and nanolithography techniques. / Camarero, Julio A.; Cheung, Chin Li; Lin, Tianwei; Johnson, John E.; Weeks, Brandon L.; Noy, Aleksandr; De Yoreo, Jim J.

2003 Nanotechnology Conference and Trade Show - Nanotech 2003. ed. / M. Laudon; B. Romanowicz. Vol. 1 2003. p. 78-80.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Camarero, JA, Cheung, CL, Lin, T, Johnson, JE, Weeks, BL, Noy, A & De Yoreo, JJ 2003, Assembly of oriented virus arrays by chemo-selective ligation methods and nanolithography techniques. in M Laudon & B Romanowicz (eds), 2003 Nanotechnology Conference and Trade Show - Nanotech 2003. vol. 1, pp. 78-80, 2003 Nanotechnology Conference and Trade Show - Nanotech 2003, San Francisco, CA, United States, 2/23/03.

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abstract = "The present work describes our ongoing efforts towards the creation of nano-scaled ordered arrays of protein/virus covalently attached to site-specific chemical linkers patterned by different nanolithograpy techniques. We will present a new and efficient solid-phase approach for the synthesis of chemically modified long alkyl-thiols. These compounds can be used to introduce chemoselective reacting groups onto gold and silicon-based surfaces. Furthermore, these modified thiols have been used to create nanometric patterns by using different nanolithography techniques. We will show that this patterns can react chemoselectively with proteins and/or virus which have been chemically or recombinantly modified to contain complementary chemical groups at specific positions thus resulting in the oriented attachment of the protein or virus to the surface.",

keywords = "Chemoselective Attachment, Cow Pea Mosaic Virus (CPMV), Dip Pen Nanolithography, Protein Arrays",

author = "Camarero, {Julio A.} and Cheung, {Chin Li} and Tianwei Lin and Johnson, {John E.} and Weeks, {Brandon L.} and Aleksandr Noy and {De Yoreo}, {Jim J.}",

year = "2003",

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AU - Camarero, Julio A.

AU - Cheung, Chin Li

AU - Lin, Tianwei

AU - Johnson, John E.

AU - Weeks, Brandon L.

AU - Noy, Aleksandr

AU - De Yoreo, Jim J.

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N2 - The present work describes our ongoing efforts towards the creation of nano-scaled ordered arrays of protein/virus covalently attached to site-specific chemical linkers patterned by different nanolithograpy techniques. We will present a new and efficient solid-phase approach for the synthesis of chemically modified long alkyl-thiols. These compounds can be used to introduce chemoselective reacting groups onto gold and silicon-based surfaces. Furthermore, these modified thiols have been used to create nanometric patterns by using different nanolithography techniques. We will show that this patterns can react chemoselectively with proteins and/or virus which have been chemically or recombinantly modified to contain complementary chemical groups at specific positions thus resulting in the oriented attachment of the protein or virus to the surface.

AB - The present work describes our ongoing efforts towards the creation of nano-scaled ordered arrays of protein/virus covalently attached to site-specific chemical linkers patterned by different nanolithograpy techniques. We will present a new and efficient solid-phase approach for the synthesis of chemically modified long alkyl-thiols. These compounds can be used to introduce chemoselective reacting groups onto gold and silicon-based surfaces. Furthermore, these modified thiols have been used to create nanometric patterns by using different nanolithography techniques. We will show that this patterns can react chemoselectively with proteins and/or virus which have been chemically or recombinantly modified to contain complementary chemical groups at specific positions thus resulting in the oriented attachment of the protein or virus to the surface.

KW - Chemoselective Attachment

KW - Cow Pea Mosaic Virus (CPMV)

KW - Dip Pen Nanolithography

KW - Protein Arrays

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