Assembly and dynamics of the bacteriophage T4 homologous recombination machinery

Jie Liu, Scott W. Morrical

Research output: Contribution to journalReview article

30 Citations (Scopus)

Abstract

Homologous recombination (HR), a process involving the physical exchange of strands between homologous or nearly homologous DNA molecules, is critical for maintaining the genetic diversity and genome stability of species. Bacteriophage T4 is one of the classic systems for studies of homologous recombination. T4 uses HR for high-frequency genetic exchanges, for homology-directed DNA repair (HDR) processes including DNA double-strand break repair, and for the initiation of DNA replication (RDR). T4 recombination proteins are expressed at high levels during T4 infection in E. coli, and share strong sequence, structural, and/or functional conservation with their counterparts in cellular organisms. Biochemical studies of T4 recombination have provided key insights on DNA strand exchange mechanisms, on the structure and function of recombination proteins, and on the coordination of recombination and DNA synthesis activities during RDR and HDR. Recent years have seen the development of detailed biochemical models for the assembly and dynamics of presynaptic filaments in the T4 recombination system, for the atomic structure of T4 UvsX recombinase, and for the roles of DNA helicases in T4 recombination. The goal of this chapter is to review these recent advances and their implications for HR and HDR mechanisms in all organisms.

Original languageEnglish (US)
Article number357
JournalVirology Journal
Volume7
DOIs
StatePublished - Dec 6 2010

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Bacteriophage T4
Homologous Recombination
Genetic Recombination
DNA Repair
DNA
Physical Phenomena
DNA Helicases
Escherichia coli Infections
Recombinases
Double-Stranded DNA Breaks
Genomic Instability
DNA Replication
Proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Assembly and dynamics of the bacteriophage T4 homologous recombination machinery. / Liu, Jie; Morrical, Scott W.

In: Virology Journal, Vol. 7, 357, 06.12.2010.

Research output: Contribution to journalReview article

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