Assays to Evaluate Toxoplasma–Macrophage Interactions

Debanjan Mukhopadhyay, Jeroen P.J. Saeij

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The obligate intracellular protozoan parasite Toxoplasma gondii can infect any nucleated cell from a warm-blooded host. However, its interaction with host macrophages plays a critical role in shaping the immune response during infection. Therefore, assessing Toxoplasma–macrophage interactions at a cellular level is important. In this chapter, we describe assays that can be used to characterize Toxoplasma–macrophage interactions. These assays can also be used to evaluate other host–pathogen interactions. We describe multiplex approaches for measuring arginase activity, indoleamine 2,3 dioxygenase activity, cell death, and parasite growth during Toxoplasma–macrophage interactions. These assays can be used to compare how different Toxoplasma strains differ in their interaction with macrophages, and we describe how to properly assess Toxoplasma strain differences in Toxoplasma–macrophage interactions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages347-370
Number of pages24
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2071
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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Keywords

  • Arginase
  • GRA15
  • IDO
  • Inflammasome
  • LDH
  • Macrophage
  • Macrophage polarization
  • MTS
  • ROP16
  • Toxoplasma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mukhopadhyay, D., & Saeij, J. P. J. (2020). Assays to Evaluate Toxoplasma–Macrophage Interactions. In Methods in Molecular Biology (pp. 347-370). (Methods in Molecular Biology; Vol. 2071). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9857-9_19