ASIC1a plays a key role in evoking the metabolic component of the exercise pressor reflex in rats

Guillaume P. Ducrocq, Joyce S. Kim, Juan A. Estrada, Marc P. Kaufman

    Research output: Contribution to journalArticle

    Abstract

    The role of the acid-sensing ion channel 1a (ASIC1a) in evoking the exercise pressor reflex is unknown, despite the fact that ASIC1a is opened by decreases in pH in the physiological range. This fact prompted us to test the hypothesis that ASIC1a plays an important role in evoking the exercise pressor reflex in decerebrated rats with freely perfused hindlimb muscles. To test this hypothesis, we measured the effect of injecting two ASIC1a blockers into the arterial supply of the triceps surae muscles on the reflex pressor responses to four maneuvers, namely 1) static contraction of the triceps surae muscles (i.e., the exercise pressor reflex), 2) calcaneal tendon stretch, 3) intra-arterial injection of lactic acid, and 4) intra-arterial injection of diprotonated phosphate. We found that the 2 ASIC1a blockers, psalmotoxin-1 (200 ng/kg) and mambalgin-1 (6.5 μg/kg), decreased the pressor responses to static contraction as well as the peak pressor responses to injection of lactic acid and diprotonated phosphate. In contrast, neither ASIC1a blocker had any effect on the pressor responses to tendon stretch. Importantly, we found that ASIC1a blockade significantly decreased the pressor response to static contraction after a latency of at least 8 s. Our results support the hypothesis that ASIC1a plays a key role in evoking the metabolic component of the exercise pressor reflex. NEW & NOTEWORTHY The role played by acid-sensing ion channel 1a (ASIC1a) in evoking the exercise pressor reflex remains unknown. In decerebrated rats with freely perfused femoral arteries, blocking ASIC1a with psalmotoxin-1 or mambalgin-1 significantly attenuated the pressor response to static contraction, lactic acid, and diprotonated phosphate injection but had no effect on the pressor response to stretch. We conclude that ASIC1a plays a key role in evoking the exercise pressor reflex by responding to contraction-induced metabolites, such as protons.

    Original languageEnglish (US)
    Pages (from-to)H78-H89
    JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
    Volume318
    Issue number1
    DOIs
    StatePublished - Jan 1 2020

    Fingerprint

    Acid Sensing Ion Channels
    Reflex
    Acid Sensing Ion Channel Blockers
    Intra-Arterial Injections
    Lactic Acid
    Phosphates
    Muscles
    Injections
    Achilles Tendon
    Femoral Artery
    Hindlimb
    Tendons
    Protons

    Keywords

    • Autonomic control
    • Mambalgin
    • Mechanoreflex
    • Metaboreflex
    • Psalmotoxin

    ASJC Scopus subject areas

    • Physiology
    • Cardiology and Cardiovascular Medicine
    • Physiology (medical)

    Cite this

    ASIC1a plays a key role in evoking the metabolic component of the exercise pressor reflex in rats. / Ducrocq, Guillaume P.; Kim, Joyce S.; Estrada, Juan A.; Kaufman, Marc P.

    In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 318, No. 1, 01.01.2020, p. H78-H89.

    Research output: Contribution to journalArticle

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