Abstract
Background: The artificially reared rat model was used successfully to study the effect of nutrition during the early postnatal period on growth and development of the neonate. Over-growth and morphologic changes of the gastrointestinal tract are known consequences of artificial rearing. The major goal of our study was to elucidate whether artificial rearing-enhanced gut development is caused by artificial diet or by gastrostomy and the artificial rearing technique itself. Methods: Suckling rats at day 8 of age underwent intragastric cannulation and were machine fed either a cow's milk-based artificial rat's milk substitute or pooled rat's milk for 4 days. Dam-fed littermates served as a control. Results: Body growth did not differ in the three experimental groups. In rats receiving rat's milk substitute, small intestinal wet weight was approximately 60% greater than in rats fed rat's milk or control rats. Additionally, the entire small intestine was approximately 20% longer in the rat's milk substitute group. Morphologically, rat's milk substitute-fed pups demonstrated significantly greater intestinal villus length and crypt depth compared with rat's milk-fed or control rats. Jejunum and mid-jejunum of the rat's milk and control groups did not differ in these parameters. Intestinal sucrase activity of rat's milk substitute-fed rats was significantly elevated compared with rat's milk-fed rats or control animals. Conclusions: These results indicate that cow's milk-based formula, not gastrostomy or artificial feeding technique, is a principal cause of the small intestine overgrowth and precocious maturation of some intestinal functions observed in artificially reared sucklings. (C) 2000 Lippincott Williams and Wilkins, Inc.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 162-169 |
| Number of pages | 8 |
| Journal | Journal of Pediatric Gastroenterology and Nutrition |
| Volume | 31 |
| Issue number | SUPPL. 2 |
| DOIs | |
| State | Published - 2000 |
| Externally published | Yes |
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Keywords
- Artificial rearing
- Gastrointestinal tract
- Infant nutrition
- Rat's milk
- Small intestine
- Suckling rats
ASJC Scopus subject areas
- Gastroenterology
- Histology
- Medicine (miscellaneous)
- Food Science
- Pediatrics, Perinatology, and Child Health
Cite this
Artificial formula induces precocious maturation of the small intestine of artificially reared suckling rats. / Dvorak, B.; McWilliam, D. L.; Williams, C. S.; Dominguez, J. A.; Machen, N. W.; McCuskey, R. S.; Philipps, Anthony F.
In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 31, No. SUPPL. 2, 2000, p. 162-169.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Artificial formula induces precocious maturation of the small intestine of artificially reared suckling rats
AU - Dvorak, B.
AU - McWilliam, D. L.
AU - Williams, C. S.
AU - Dominguez, J. A.
AU - Machen, N. W.
AU - McCuskey, R. S.
AU - Philipps, Anthony F
PY - 2000
Y1 - 2000
N2 - Background: The artificially reared rat model was used successfully to study the effect of nutrition during the early postnatal period on growth and development of the neonate. Over-growth and morphologic changes of the gastrointestinal tract are known consequences of artificial rearing. The major goal of our study was to elucidate whether artificial rearing-enhanced gut development is caused by artificial diet or by gastrostomy and the artificial rearing technique itself. Methods: Suckling rats at day 8 of age underwent intragastric cannulation and were machine fed either a cow's milk-based artificial rat's milk substitute or pooled rat's milk for 4 days. Dam-fed littermates served as a control. Results: Body growth did not differ in the three experimental groups. In rats receiving rat's milk substitute, small intestinal wet weight was approximately 60% greater than in rats fed rat's milk or control rats. Additionally, the entire small intestine was approximately 20% longer in the rat's milk substitute group. Morphologically, rat's milk substitute-fed pups demonstrated significantly greater intestinal villus length and crypt depth compared with rat's milk-fed or control rats. Jejunum and mid-jejunum of the rat's milk and control groups did not differ in these parameters. Intestinal sucrase activity of rat's milk substitute-fed rats was significantly elevated compared with rat's milk-fed rats or control animals. Conclusions: These results indicate that cow's milk-based formula, not gastrostomy or artificial feeding technique, is a principal cause of the small intestine overgrowth and precocious maturation of some intestinal functions observed in artificially reared sucklings. (C) 2000 Lippincott Williams and Wilkins, Inc.
AB - Background: The artificially reared rat model was used successfully to study the effect of nutrition during the early postnatal period on growth and development of the neonate. Over-growth and morphologic changes of the gastrointestinal tract are known consequences of artificial rearing. The major goal of our study was to elucidate whether artificial rearing-enhanced gut development is caused by artificial diet or by gastrostomy and the artificial rearing technique itself. Methods: Suckling rats at day 8 of age underwent intragastric cannulation and were machine fed either a cow's milk-based artificial rat's milk substitute or pooled rat's milk for 4 days. Dam-fed littermates served as a control. Results: Body growth did not differ in the three experimental groups. In rats receiving rat's milk substitute, small intestinal wet weight was approximately 60% greater than in rats fed rat's milk or control rats. Additionally, the entire small intestine was approximately 20% longer in the rat's milk substitute group. Morphologically, rat's milk substitute-fed pups demonstrated significantly greater intestinal villus length and crypt depth compared with rat's milk-fed or control rats. Jejunum and mid-jejunum of the rat's milk and control groups did not differ in these parameters. Intestinal sucrase activity of rat's milk substitute-fed rats was significantly elevated compared with rat's milk-fed rats or control animals. Conclusions: These results indicate that cow's milk-based formula, not gastrostomy or artificial feeding technique, is a principal cause of the small intestine overgrowth and precocious maturation of some intestinal functions observed in artificially reared sucklings. (C) 2000 Lippincott Williams and Wilkins, Inc.
KW - Artificial rearing
KW - Gastrointestinal tract
KW - Infant nutrition
KW - Rat's milk
KW - Small intestine
KW - Suckling rats
UR - http://www.scopus.com/inward/record.url?scp=0033853189&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033853189&partnerID=8YFLogxK
U2 - 10.1097/00005176-200008000-00013
DO - 10.1097/00005176-200008000-00013
M3 - Article
C2 - 10941968
AN - SCOPUS:0033853189
VL - 31
SP - 162
EP - 169
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
SN - 0277-2116
IS - SUPPL. 2
ER -