Article Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice

Feng Hu, Yu Yan, Chu wang Wang, Yu Liu, Jing jing Wang, Fang Zhou, Qing hai Zeng, Xiao Zhou, Jia Chen, Aijun Wang, Jian da Zhou

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on human fibroblasts and skin wound healing in Kunming male mice and to explore the putative molecular mechanism. Methods: Primary human skin fibroblasts were cultured. The viability of fibroblasts treated with 0, 10, 20, 40, 80, and 160 μg/mL of GL-PS, respectively were detected by 3–4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-Htetrazolium bromide (MTT). The migration ability of fibroblasts treated with 0, 10, 20, and 40 μg/mL of GL-PS were measured by transwell assay. The secretion of the C-terminal peptide of procollagen type I (CICP) and transforming growth factor-β1 (TGF-β1) in the cell supernatant was tested by enzyme-linked immunosorbent assay. The expression of β-catenin was detected by Western blot. Furthermore, the Kunming mouse model with full-layer skin resection trauma was established, and was treated with 10, 20, and 40 mg/mL of GL-PS, respectively as external use. The size of the wound was measured daily, complete healing time in each group was recorded and the percentage of wound contraction was calculated. Results: Compared with the control group, 10, 20, and 40 μg/mL of GL-PS significantly increased the viability of fibroblasts, promoted the migration ability of fibroblasts, and up-regulated the expressions of CICP and TGF-β1 in fibroblasts (Plt;0.05 or Plt;0.01). The expression of β-catenin in fibroblasts treated with 20 and 40 μg/mL of GL-PS was significantly higher than that of the control group (Plt;0.01). Furthermore, after external use of 10, 20, and 40 mg/mL of GL-PS, the rates of wound healing in mice were significantly higher and the wound healing time was significantly less than the control group (Plt;0.05 or Plt;0.01). Conclusion: A certain concentration of GL-PS may promote wound healing via activation of the Wnt/β-catenin signaling pathway and up-regulation of TGF-β1, which might serve as a promising source of skin wound healing.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalChinese Journal of Integrative Medicine
Volume25
Issue number3
DOIs
StatePublished - Mar 1 2019

Fingerprint

Reishi
Wound Healing
Polysaccharides
Fibroblasts
Skin
Catenins
Transforming Growth Factors
Control Groups
Wounds and Injuries
Wnt Signaling Pathway
Bromides
Up-Regulation
Western Blotting
Enzyme-Linked Immunosorbent Assay

Keywords

  • Ganoderma lucidum polysaccharides
  • human fibroblast
  • transforming growth factor-β1
  • Wnt/β-catenin signaling pathway
  • wound healing

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Pharmacology (medical)

Cite this

Article Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice. / Hu, Feng; Yan, Yu; Wang, Chu wang; Liu, Yu; Wang, Jing jing; Zhou, Fang; Zeng, Qing hai; Zhou, Xiao; Chen, Jia; Wang, Aijun; Zhou, Jian da.

In: Chinese Journal of Integrative Medicine, Vol. 25, No. 3, 01.03.2019, p. 203-209.

Research output: Contribution to journalArticle

Hu, Feng ; Yan, Yu ; Wang, Chu wang ; Liu, Yu ; Wang, Jing jing ; Zhou, Fang ; Zeng, Qing hai ; Zhou, Xiao ; Chen, Jia ; Wang, Aijun ; Zhou, Jian da. / Article Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice. In: Chinese Journal of Integrative Medicine. 2019 ; Vol. 25, No. 3. pp. 203-209.
@article{9871db4b682e4a3e9b4b0fd4219cf15a,
title = "Article Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice",
abstract = "Objective: To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on human fibroblasts and skin wound healing in Kunming male mice and to explore the putative molecular mechanism. Methods: Primary human skin fibroblasts were cultured. The viability of fibroblasts treated with 0, 10, 20, 40, 80, and 160 μg/mL of GL-PS, respectively were detected by 3–4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-Htetrazolium bromide (MTT). The migration ability of fibroblasts treated with 0, 10, 20, and 40 μg/mL of GL-PS were measured by transwell assay. The secretion of the C-terminal peptide of procollagen type I (CICP) and transforming growth factor-β1 (TGF-β1) in the cell supernatant was tested by enzyme-linked immunosorbent assay. The expression of β-catenin was detected by Western blot. Furthermore, the Kunming mouse model with full-layer skin resection trauma was established, and was treated with 10, 20, and 40 mg/mL of GL-PS, respectively as external use. The size of the wound was measured daily, complete healing time in each group was recorded and the percentage of wound contraction was calculated. Results: Compared with the control group, 10, 20, and 40 μg/mL of GL-PS significantly increased the viability of fibroblasts, promoted the migration ability of fibroblasts, and up-regulated the expressions of CICP and TGF-β1 in fibroblasts (Plt;0.05 or Plt;0.01). The expression of β-catenin in fibroblasts treated with 20 and 40 μg/mL of GL-PS was significantly higher than that of the control group (Plt;0.01). Furthermore, after external use of 10, 20, and 40 mg/mL of GL-PS, the rates of wound healing in mice were significantly higher and the wound healing time was significantly less than the control group (Plt;0.05 or Plt;0.01). Conclusion: A certain concentration of GL-PS may promote wound healing via activation of the Wnt/β-catenin signaling pathway and up-regulation of TGF-β1, which might serve as a promising source of skin wound healing.",
keywords = "Ganoderma lucidum polysaccharides, human fibroblast, transforming growth factor-β1, Wnt/β-catenin signaling pathway, wound healing",
author = "Feng Hu and Yu Yan and Wang, {Chu wang} and Yu Liu and Wang, {Jing jing} and Fang Zhou and Zeng, {Qing hai} and Xiao Zhou and Jia Chen and Aijun Wang and Zhou, {Jian da}",
year = "2019",
month = "3",
day = "1",
doi = "10.1007/s11655-018-3060-9",
language = "English (US)",
volume = "25",
pages = "203--209",
journal = "Chinese Journal of Integrative Medicine",
issn = "1672-0415",
publisher = "Chinese Journal of Integrated Traditional and Western Medicine Press",
number = "3",

}

TY - JOUR

T1 - Article Effect and Mechanism of Ganoderma lucidum Polysaccharides on Human Fibroblasts and Skin Wound Healing in Mice

AU - Hu, Feng

AU - Yan, Yu

AU - Wang, Chu wang

AU - Liu, Yu

AU - Wang, Jing jing

AU - Zhou, Fang

AU - Zeng, Qing hai

AU - Zhou, Xiao

AU - Chen, Jia

AU - Wang, Aijun

AU - Zhou, Jian da

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Objective: To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on human fibroblasts and skin wound healing in Kunming male mice and to explore the putative molecular mechanism. Methods: Primary human skin fibroblasts were cultured. The viability of fibroblasts treated with 0, 10, 20, 40, 80, and 160 μg/mL of GL-PS, respectively were detected by 3–4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-Htetrazolium bromide (MTT). The migration ability of fibroblasts treated with 0, 10, 20, and 40 μg/mL of GL-PS were measured by transwell assay. The secretion of the C-terminal peptide of procollagen type I (CICP) and transforming growth factor-β1 (TGF-β1) in the cell supernatant was tested by enzyme-linked immunosorbent assay. The expression of β-catenin was detected by Western blot. Furthermore, the Kunming mouse model with full-layer skin resection trauma was established, and was treated with 10, 20, and 40 mg/mL of GL-PS, respectively as external use. The size of the wound was measured daily, complete healing time in each group was recorded and the percentage of wound contraction was calculated. Results: Compared with the control group, 10, 20, and 40 μg/mL of GL-PS significantly increased the viability of fibroblasts, promoted the migration ability of fibroblasts, and up-regulated the expressions of CICP and TGF-β1 in fibroblasts (Plt;0.05 or Plt;0.01). The expression of β-catenin in fibroblasts treated with 20 and 40 μg/mL of GL-PS was significantly higher than that of the control group (Plt;0.01). Furthermore, after external use of 10, 20, and 40 mg/mL of GL-PS, the rates of wound healing in mice were significantly higher and the wound healing time was significantly less than the control group (Plt;0.05 or Plt;0.01). Conclusion: A certain concentration of GL-PS may promote wound healing via activation of the Wnt/β-catenin signaling pathway and up-regulation of TGF-β1, which might serve as a promising source of skin wound healing.

AB - Objective: To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on human fibroblasts and skin wound healing in Kunming male mice and to explore the putative molecular mechanism. Methods: Primary human skin fibroblasts were cultured. The viability of fibroblasts treated with 0, 10, 20, 40, 80, and 160 μg/mL of GL-PS, respectively were detected by 3–4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2-Htetrazolium bromide (MTT). The migration ability of fibroblasts treated with 0, 10, 20, and 40 μg/mL of GL-PS were measured by transwell assay. The secretion of the C-terminal peptide of procollagen type I (CICP) and transforming growth factor-β1 (TGF-β1) in the cell supernatant was tested by enzyme-linked immunosorbent assay. The expression of β-catenin was detected by Western blot. Furthermore, the Kunming mouse model with full-layer skin resection trauma was established, and was treated with 10, 20, and 40 mg/mL of GL-PS, respectively as external use. The size of the wound was measured daily, complete healing time in each group was recorded and the percentage of wound contraction was calculated. Results: Compared with the control group, 10, 20, and 40 μg/mL of GL-PS significantly increased the viability of fibroblasts, promoted the migration ability of fibroblasts, and up-regulated the expressions of CICP and TGF-β1 in fibroblasts (Plt;0.05 or Plt;0.01). The expression of β-catenin in fibroblasts treated with 20 and 40 μg/mL of GL-PS was significantly higher than that of the control group (Plt;0.01). Furthermore, after external use of 10, 20, and 40 mg/mL of GL-PS, the rates of wound healing in mice were significantly higher and the wound healing time was significantly less than the control group (Plt;0.05 or Plt;0.01). Conclusion: A certain concentration of GL-PS may promote wound healing via activation of the Wnt/β-catenin signaling pathway and up-regulation of TGF-β1, which might serve as a promising source of skin wound healing.

KW - Ganoderma lucidum polysaccharides

KW - human fibroblast

KW - transforming growth factor-β1

KW - Wnt/β-catenin signaling pathway

KW - wound healing

UR - http://www.scopus.com/inward/record.url?scp=85058379458&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058379458&partnerID=8YFLogxK

U2 - 10.1007/s11655-018-3060-9

DO - 10.1007/s11655-018-3060-9

M3 - Article

VL - 25

SP - 203

EP - 209

JO - Chinese Journal of Integrative Medicine

JF - Chinese Journal of Integrative Medicine

SN - 1672-0415

IS - 3

ER -