Abstract
Arrays of Carbon nanofibers (CNFs) harness the advantages of individual CNF as well the collective property of assemblies, which made them promising materials in biosensing and tissue engineering or implantation. Here, we report two studies to explore the applications of vertically aligned CNFs. First, a nanoelectrode array (NEA) based on vertically aligned CNFs embedded in SiO 2 is used for ultrasensitive DNA detection. Oligonucleotide probes are selectively functionalized at the open ends of the CNFs and specifically hybridized with oligonucleotide targets. The guanine groups are employed as the signal moieties in the electrochemical measurements. Ru(bpy)3 2+ mediator is used to further amplify the guanine oxidation signal. The hybridization of less than ~1000 molecules of PCR amplified DNA targets are detected electrochemically by combining the CNF nanoelectrode array with the Ru(bpy)3 2+ amplification mechanism. Second, the SiO 2 matrix was etched back to produce needle-like protruding nanoelectrode arrays to be used as cell interfacing fibers for investigating gene transfection, electrical stimulation and detection of cellular processes. Our goal is to take advantage of the nanostructure of CNFs for unconventional biomolecular studies requiring ultrahigh sensitivity, high-degree of miniaturization and selective biofunctionalization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 35-43 |
| Number of pages | 9 |
| Journal | Bio-Medical Materials and Engineering |
| Volume | 19 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2009 |
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Keywords
- Carbon nanofibers
- Cell scaffold
- DNA sensors
- Vertically aligned nanoelectrode array
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
Cite this
Arrays of carbon nanofibers as a platform for biosensing at the molecular level and for tissue engineering and implantation. / Koehne, Jessica E.; Chen, Hua; Cassell, Alan; Liu, Gang-yu; Li, Jun; Meyyappan, M.
In: Bio-Medical Materials and Engineering, Vol. 19, No. 1, 2009, p. 35-43.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Arrays of carbon nanofibers as a platform for biosensing at the molecular level and for tissue engineering and implantation
AU - Koehne, Jessica E.
AU - Chen, Hua
AU - Cassell, Alan
AU - Liu, Gang-yu
AU - Li, Jun
AU - Meyyappan, M.
PY - 2009
Y1 - 2009
N2 - Arrays of Carbon nanofibers (CNFs) harness the advantages of individual CNF as well the collective property of assemblies, which made them promising materials in biosensing and tissue engineering or implantation. Here, we report two studies to explore the applications of vertically aligned CNFs. First, a nanoelectrode array (NEA) based on vertically aligned CNFs embedded in SiO 2 is used for ultrasensitive DNA detection. Oligonucleotide probes are selectively functionalized at the open ends of the CNFs and specifically hybridized with oligonucleotide targets. The guanine groups are employed as the signal moieties in the electrochemical measurements. Ru(bpy)3 2+ mediator is used to further amplify the guanine oxidation signal. The hybridization of less than ~1000 molecules of PCR amplified DNA targets are detected electrochemically by combining the CNF nanoelectrode array with the Ru(bpy)3 2+ amplification mechanism. Second, the SiO 2 matrix was etched back to produce needle-like protruding nanoelectrode arrays to be used as cell interfacing fibers for investigating gene transfection, electrical stimulation and detection of cellular processes. Our goal is to take advantage of the nanostructure of CNFs for unconventional biomolecular studies requiring ultrahigh sensitivity, high-degree of miniaturization and selective biofunctionalization.
AB - Arrays of Carbon nanofibers (CNFs) harness the advantages of individual CNF as well the collective property of assemblies, which made them promising materials in biosensing and tissue engineering or implantation. Here, we report two studies to explore the applications of vertically aligned CNFs. First, a nanoelectrode array (NEA) based on vertically aligned CNFs embedded in SiO 2 is used for ultrasensitive DNA detection. Oligonucleotide probes are selectively functionalized at the open ends of the CNFs and specifically hybridized with oligonucleotide targets. The guanine groups are employed as the signal moieties in the electrochemical measurements. Ru(bpy)3 2+ mediator is used to further amplify the guanine oxidation signal. The hybridization of less than ~1000 molecules of PCR amplified DNA targets are detected electrochemically by combining the CNF nanoelectrode array with the Ru(bpy)3 2+ amplification mechanism. Second, the SiO 2 matrix was etched back to produce needle-like protruding nanoelectrode arrays to be used as cell interfacing fibers for investigating gene transfection, electrical stimulation and detection of cellular processes. Our goal is to take advantage of the nanostructure of CNFs for unconventional biomolecular studies requiring ultrahigh sensitivity, high-degree of miniaturization and selective biofunctionalization.
KW - Carbon nanofibers
KW - Cell scaffold
KW - DNA sensors
KW - Vertically aligned nanoelectrode array
UR - http://www.scopus.com/inward/record.url?scp=66249102271&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66249102271&partnerID=8YFLogxK
U2 - 10.3233/BME-2009-0561
DO - 10.3233/BME-2009-0561
M3 - Article
C2 - 19458444
AN - SCOPUS:66249102271
VL - 19
SP - 35
EP - 43
JO - Bio-Medical Materials and Engineering
JF - Bio-Medical Materials and Engineering
SN - 0959-2989
IS - 1
ER -