Aromatic amino acid catabolism by lactococci

S. Gao, D. H. Oh, J. R. Broadbent, M. E. Johnson, Bart C Weimer, J. L. Steele

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

While catabolism of amino acids is believed to play an important role in cheese flavor development, the pathways present in cheese microflora are poorly understood. To determine the pathways of aromatic amino acid catabolism in lactococci and effects of Cheddar cheese ripening conditions on catabolic enzymes and products, eight starter lactococcal strains were screened. Cell-free extracts prepared from these strains were found to contain an α-ketoglutarate-dependent aminotransferase activity with tryptophan, tyrosine and phenylalanine. Tryptophan, tyrosine and phenylalanine amino-transferase specific activities (μmol product formed/mg protein/min) ranged from 0.30 to 2.8 10-3, 0.93 to 7.3 10-3 and 1.5 to 7.2 10-3, respectively. Metabolites produced from tryptophan by a cell-free extract of Lactococcus lactis S3 were indolepyruvic acid, indoleacetic acid and indole-3-aldehyde. Indoleacetic acid and indole-3-aldehyde can form spontaneously from indolepyruvic acid under the conditions employed. A defined medium was used to determine whether the aminotransferase(s) was expressed and which metabolite(s) accumulate under conditions that simulated those of ripening Cheddar cheese in terms of pH, salt, temperature and carbohydrate starvation. The results indicated that the aminotransferase(s) was expressed and stable under these conditions. The tryptophan metabolites that accumulated were determined to be strain-specific.

Original languageEnglish (US)
Pages (from-to)371-381
Number of pages11
JournalLait
Volume77
Issue number3
StatePublished - May 1997
Externally publishedYes

Keywords

  • Aminotransferase
  • Aromatic amino acid
  • Catabolism
  • Cheese flavor
  • Lactococcus

ASJC Scopus subject areas

  • Food Science

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    Gao, S., Oh, D. H., Broadbent, J. R., Johnson, M. E., Weimer, B. C., & Steele, J. L. (1997). Aromatic amino acid catabolism by lactococci. Lait, 77(3), 371-381.