Are lipid phase transitions responsible for chilling damage in human platelets?

John H. Crowe, Fern Tablin, Nelly Tsvetkova, Ann E. Oliver, Naomi Walker, Lois M. Crowe

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

In previous studies we have proposed that the well-known chilling- induced activation of human blood platelets can be ascribed at least in part to a thermotropic phase transition in membrane lipids. The evidence that this is the case is reviewed and amplified in this review, followed by an examination of the available physical data concerning phase transitions in lipid mixtures that mimic the mixture found in platelet membranes. Assuming complete mixing at all temperatures and equal contributions of the members of the mixture to the phase transition, the lipid mixture found in platelets should give values for T(m) ranging from about 1°C to about 16°C, depending on the isomers present in the mixture. (The former value is not in agreement with the observed T(m), but the latter is in excellent agreement.) However, examination of the phase diagram for a binary pair of lipids found in platelet membranes shows that ideal mixing almost certainly does not occur; instead of a linear phase diagram, a convex one was obtained. This shape for the phase diagram, which would displace T(m) to an unexpectedly elevated temperature, is in agreement with previously published phase diagrams for mixtures of this type. The prediction, based on thermodynamic properties of lipids found in the platelets, is that T(m) will be displaced upward in more complex mixtures of the composition found in platelets, a prediction that requires experimental testing.

Original languageEnglish (US)
Pages (from-to)180-191
Number of pages12
JournalCryobiology
Volume38
Issue number3
DOIs
StatePublished - May 1999

Fingerprint

Chilling
Phase Transition
phase transition
Platelets
Blood Platelets
Phase transitions
Lipids
Phase diagrams
lipids
Membranes
Temperature
blood platelets
Membrane Lipids
prediction
Complex Mixtures
Thermodynamics
Isomers
Physical Examination
cold treatment
thermodynamics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Crowe, J. H., Tablin, F., Tsvetkova, N., Oliver, A. E., Walker, N., & Crowe, L. M. (1999). Are lipid phase transitions responsible for chilling damage in human platelets? Cryobiology, 38(3), 180-191. https://doi.org/10.1006/cryo.1998.2137

Are lipid phase transitions responsible for chilling damage in human platelets? / Crowe, John H.; Tablin, Fern; Tsvetkova, Nelly; Oliver, Ann E.; Walker, Naomi; Crowe, Lois M.

In: Cryobiology, Vol. 38, No. 3, 05.1999, p. 180-191.

Research output: Contribution to journalArticle

Crowe, JH, Tablin, F, Tsvetkova, N, Oliver, AE, Walker, N & Crowe, LM 1999, 'Are lipid phase transitions responsible for chilling damage in human platelets?', Cryobiology, vol. 38, no. 3, pp. 180-191. https://doi.org/10.1006/cryo.1998.2137
Crowe, John H. ; Tablin, Fern ; Tsvetkova, Nelly ; Oliver, Ann E. ; Walker, Naomi ; Crowe, Lois M. / Are lipid phase transitions responsible for chilling damage in human platelets?. In: Cryobiology. 1999 ; Vol. 38, No. 3. pp. 180-191.
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