Archaeal RadA protein binds DNA as both helical filaments and octameric rings

Shixin Yang, Xiong Yu, Erica M. Seitz, Stephen C. Kowalczykowski, Edward H. Egelman

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The Escherichia coli RecA protein has been a model for understanding homologous eukaryotic recombination proteins such as Rad51. The active form of both RecA and Rad51 appear to be helical filaments polymerized on DNA, in which an unusual helical structure is induced in the DNA. Surprisingly, the human meiosis-specific homolog of RecA, Dmc1, has thus far only been observed to bind DNA as an octameric ring. Sequence analysis and biochemical studies have shown that archaeal RadA proteins are more closely related to Rad51 and Dmc1 than the bacterial RecA proteins. We find that the Sulfolobus solfataricus RadA protein binds DNA in the absence of nucleotide cofactor as an octameric ring and in the presence of ATP as a helical filament. Since it is likely that RadA is closely related to a common ancestral protein of both Rad51 and Dmc1, the two DNA-binding forms of RadA may provide insight into the divergence that has taken place between Rad51 and Dmc1.

Original languageEnglish (US)
Pages (from-to)1077-1085
Number of pages9
JournalJournal of Molecular Biology
Volume314
Issue number5
DOIs
StatePublished - Dec 14 2001

Fingerprint

Rec A Recombinases
DNA
Sulfolobus solfataricus
Proteins
Bacterial Proteins
Escherichia coli Proteins
Homologous Recombination
Meiosis
Sequence Analysis
Nucleotides
Adenosine Triphosphate
Archaeal RadA protein

Keywords

  • Electron microscopy
  • Image analysis
  • Protein-DNA complexes
  • Recombination
  • Ring proteins

ASJC Scopus subject areas

  • Virology

Cite this

Yang, S., Yu, X., Seitz, E. M., Kowalczykowski, S. C., & Egelman, E. H. (2001). Archaeal RadA protein binds DNA as both helical filaments and octameric rings. Journal of Molecular Biology, 314(5), 1077-1085. https://doi.org/10.1006/jmbi.2000.5213

Archaeal RadA protein binds DNA as both helical filaments and octameric rings. / Yang, Shixin; Yu, Xiong; Seitz, Erica M.; Kowalczykowski, Stephen C.; Egelman, Edward H.

In: Journal of Molecular Biology, Vol. 314, No. 5, 14.12.2001, p. 1077-1085.

Research output: Contribution to journalArticle

Yang, S, Yu, X, Seitz, EM, Kowalczykowski, SC & Egelman, EH 2001, 'Archaeal RadA protein binds DNA as both helical filaments and octameric rings', Journal of Molecular Biology, vol. 314, no. 5, pp. 1077-1085. https://doi.org/10.1006/jmbi.2000.5213
Yang, Shixin ; Yu, Xiong ; Seitz, Erica M. ; Kowalczykowski, Stephen C. ; Egelman, Edward H. / Archaeal RadA protein binds DNA as both helical filaments and octameric rings. In: Journal of Molecular Biology. 2001 ; Vol. 314, No. 5. pp. 1077-1085.
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