Arbovirus high fidelity variant loses fitness in mosquitoes and mice

Lark L Schneider, Yasnee Beeharry, Antonio V. Bordería, Hervé Blanc, Marco Vignuzzi

Research output: Contribution to journalArticlepeer-review

170 Scopus citations

Abstract

The error rate of RNA-dependent RNA polymerases (RdRp) affects the mutation frequency in a population of viral RNAs. Using chikungunya virus (CHIKV), we describe a unique arbovirus fidelity variant with a single C483Y amino acid change in the nsP4 RdRp that increases replication fidelity and generates populations with reduced genetic diversity. In mosquitoes, high fidelity CHIKV presents lower infection and dissemination titers than wild type. In newborn mice, high fidelity CHIKV produces truncated viremias and lower organ titers. These results indicate that increased replication fidelity and reduced genetic diversity negatively impact arbovirus fitness in invertebrate and vertebrate hosts.

Original languageEnglish (US)
Pages (from-to)16038-16043
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number38
DOIs
StatePublished - Oct 20 2011
Externally publishedYes

ASJC Scopus subject areas

  • General

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