Arabidopsis receptor-like cytoplasmic kinase BIK1: Purification, crystallization and X-ray diffraction analysis

Neeraj K. Lal, Andrew J Fisher, Savithramma P. Dinesh-Kumar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Receptor-like cytoplasmic kinases (RLCKs) in Arabidopsis play a central role in the integration of signaling input from various growth and immune signaling pathways. BOTRYTIS-INDUCED KINASE 1 (BIK1), belonging to the RLCK family, is an important player in defense against bacterial and fungal pathogens and in ethylene and brassinosteroid hormone signaling. In this study, the purification and crystallization of a first member of the class VI family of RLCK proteins, BIK1, are reported. BIK1 was crystallized using the microbatch-under-oil method. X-ray diffraction data were collected to 2.35 Å resolution. The crystals belonged to the monoclinic space group C2, with two monomers per asymmetric unit.The purification, crystallization and X-ray diffraction analysis of the Arabidopsis receptor-like cytoplasmic kinase BIK1, which plays an important role in growth and immune signaling pathways, are described.

Original languageEnglish (US)
Pages (from-to)738-742
Number of pages5
JournalActa Crystallographica Section F:Structural Biology Communications
Volume72
Issue number10
DOIs
StatePublished - Oct 1 2016

Fingerprint

Botrytis
Cytoplasmic and Nuclear Receptors
Crystallization
Arabidopsis
purification
X-Ray Diffraction
X ray diffraction analysis
Purification
Phosphotransferases
crystallization
diffraction
x rays
pathogens
hormones
Brassinosteroids
ethylene
monomers
oils
proteins
Pathogens

Keywords

  • Arabidopsis
  • BIK1
  • BOTRYTIS-INDUCED KINASE 1
  • PAMP-triggered immunity
  • receptor-like cytoplasmic kinases
  • receptor-like kinase
  • RLCK

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Arabidopsis receptor-like cytoplasmic kinase BIK1 : Purification, crystallization and X-ray diffraction analysis. / Lal, Neeraj K.; Fisher, Andrew J; Dinesh-Kumar, Savithramma P.

In: Acta Crystallographica Section F:Structural Biology Communications, Vol. 72, No. 10, 01.10.2016, p. 738-742.

Research output: Contribution to journalArticle

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