Abstract
A study of the apurinic/apyrimidinic (AP) endonuclease activities of a mutant line of CHO cells, EM9, and its parental cell line, AA8, was undertaken to determine if the defective DNA repair exhibited by the mutant cell line after exposure to ethyl methanesulfonate was due to a defective AP endonuclease activity. Phosphocellulose chromatography of cell extracts resolved the AP endonuclease activities of both cell lines into two peaks as seen previously in mouse and human cells. No difference was found between the mutant and parental cell lines in the relative amount of AP endonuclease activity present in the two peaks.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 41-44 |
| Number of pages | 4 |
| Journal | Mutation Research Letters |
| Volume | 141 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Toxicology
- Medicine(all)
Cite this
Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9. / La Belle, M.; Linn, S.; Thompson, L. H.
In: Mutation Research Letters, Vol. 141, No. 1, 1984, p. 41-44.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9
AU - La Belle, M.
AU - Linn, S.
AU - Thompson, L. H.
PY - 1984
Y1 - 1984
N2 - A study of the apurinic/apyrimidinic (AP) endonuclease activities of a mutant line of CHO cells, EM9, and its parental cell line, AA8, was undertaken to determine if the defective DNA repair exhibited by the mutant cell line after exposure to ethyl methanesulfonate was due to a defective AP endonuclease activity. Phosphocellulose chromatography of cell extracts resolved the AP endonuclease activities of both cell lines into two peaks as seen previously in mouse and human cells. No difference was found between the mutant and parental cell lines in the relative amount of AP endonuclease activity present in the two peaks.
AB - A study of the apurinic/apyrimidinic (AP) endonuclease activities of a mutant line of CHO cells, EM9, and its parental cell line, AA8, was undertaken to determine if the defective DNA repair exhibited by the mutant cell line after exposure to ethyl methanesulfonate was due to a defective AP endonuclease activity. Phosphocellulose chromatography of cell extracts resolved the AP endonuclease activities of both cell lines into two peaks as seen previously in mouse and human cells. No difference was found between the mutant and parental cell lines in the relative amount of AP endonuclease activity present in the two peaks.
UR - http://www.scopus.com/inward/record.url?scp=0021146987&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021146987&partnerID=8YFLogxK
U2 - 10.1016/0165-7992(84)90035-6
DO - 10.1016/0165-7992(84)90035-6
M3 - Article
C2 - 6482894
AN - SCOPUS:0021146987
VL - 141
SP - 41
EP - 44
JO - Mutation Research Letters
JF - Mutation Research Letters
SN - 0165-7992
IS - 1
ER -