Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9

M. La Belle, S. Linn, L. H. Thompson

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18 Citations (Scopus)

Abstract

A study of the apurinic/apyrimidinic (AP) endonuclease activities of a mutant line of CHO cells, EM9, and its parental cell line, AA8, was undertaken to determine if the defective DNA repair exhibited by the mutant cell line after exposure to ethyl methanesulfonate was due to a defective AP endonuclease activity. Phosphocellulose chromatography of cell extracts resolved the AP endonuclease activities of both cell lines into two peaks as seen previously in mouse and human cells. No difference was found between the mutant and parental cell lines in the relative amount of AP endonuclease activity present in the two peaks.

Original languageEnglish (US)
Pages (from-to)41-44
Number of pages4
JournalMutation Research Letters
Volume141
Issue number1
DOIs
StatePublished - 1984
Externally publishedYes

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DNA-(Apurinic or Apyrimidinic Site) Lyase
Ethyl Methanesulfonate
CHO Cells
Endonucleases
Cells
Cell Line
Cell Extracts
DNA Repair
Chromatography
Repair
DNA

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

Cite this

Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9. / La Belle, M.; Linn, S.; Thompson, L. H.

In: Mutation Research Letters, Vol. 141, No. 1, 1984, p. 41-44.

Research output: Contribution to journalArticle

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