Aptamer-Containing surfaces for selective capture OF CD4 expressing cells

Qing Zhou, Ying Liu, Dong Sik Shin, Jaime Silangcruz, Nazgul Tuleuova, Alexander Revzin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aptamers have recently emerged as an excellent alternative to antibodies because of their inherent stability and ease of modification. In this paper, we describe the development of an aptamer-based surface for capture of cells expressing CD4 antigen. The glass or silicon surfaces were modified with amine-terminated silanes and then modified with thiolated RNA aptamer against CD4. Modification of the surface was first characterized by ellipsometry to demonstrate assembly of biointerface components and to show specific capture of recombinant CD4 protein. Subsequently, surfaces were challenged with model lymphocytes (cell lines) that were either positive or negative for CD4 antigen. Our experiments show that aptamer-functionalized surfaces have similar capture efficiency to substrates containing anti-CD4 antibody. To mimick capture of specific T-cells from a complex cell mixture, aptamer-modified surfaces were exposed to binary mixtures containing Molt-3 cells (CD4+) spiked into Daudi B cells (CD4-). 94% purity of CD4 cells was observed on aptamer-containing surfaces from an initial fraction of 15% of CD4. Given the importance of CD4 cell enumeration in HIV/AIDS diagnosis and monitoring, aptamer-based devices may offer an opportunity for novel cell detection strategies and may yield more robust and less expensive blood analysis devices in the future.

Original languageEnglish (US)
Pages (from-to)12544-12549
Number of pages6
JournalLangmuir
Volume28
Issue number34
DOIs
StatePublished - Aug 28 2012

Fingerprint

cells
CD4 Antigens
Cells
antigens
Antigens
antibodies
Antibodies
acquired immunodeficiency syndrome
Nucleotide Aptamers
Silanes
Recombinant proteins
enumeration
human immunodeficiency virus
T-cells
Lymphocytes
lymphocytes
Ellipsometry
Silicon
Binary mixtures
RNA

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Zhou, Q., Liu, Y., Shin, D. S., Silangcruz, J., Tuleuova, N., & Revzin, A. (2012). Aptamer-Containing surfaces for selective capture OF CD4 expressing cells. Langmuir, 28(34), 12544-12549. https://doi.org/10.1021/la2050338

Aptamer-Containing surfaces for selective capture OF CD4 expressing cells. / Zhou, Qing; Liu, Ying; Shin, Dong Sik; Silangcruz, Jaime; Tuleuova, Nazgul; Revzin, Alexander.

In: Langmuir, Vol. 28, No. 34, 28.08.2012, p. 12544-12549.

Research output: Contribution to journalArticle

Zhou, Q, Liu, Y, Shin, DS, Silangcruz, J, Tuleuova, N & Revzin, A 2012, 'Aptamer-Containing surfaces for selective capture OF CD4 expressing cells', Langmuir, vol. 28, no. 34, pp. 12544-12549. https://doi.org/10.1021/la2050338
Zhou Q, Liu Y, Shin DS, Silangcruz J, Tuleuova N, Revzin A. Aptamer-Containing surfaces for selective capture OF CD4 expressing cells. Langmuir. 2012 Aug 28;28(34):12544-12549. https://doi.org/10.1021/la2050338
Zhou, Qing ; Liu, Ying ; Shin, Dong Sik ; Silangcruz, Jaime ; Tuleuova, Nazgul ; Revzin, Alexander. / Aptamer-Containing surfaces for selective capture OF CD4 expressing cells. In: Langmuir. 2012 ; Vol. 28, No. 34. pp. 12544-12549.
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