Aptamer-based SERRS sensor for thrombin detection

Hansang Cho, Brian R. Baker, Sebastian Wachsmann-Hogiu, Cynthia V. Pagba, Ted A. Laurence, Stephen M. Lane, Luke P. Lee, Jeffrey B H Tok

Research output: Contribution to journalArticle

167 Scopus citations

Abstract

We describe an aptamer-based surface enhanced resonance Raman scattering (SERRS) sensor with high sensitivity, specificity, and stability for the detection of a coagulation protein, human α-thrombin. The sensor achieves high sensitivity and a limit of detection of 100 pM by monitoring the SERRS signal change upon the single-step of thrombin binding to immobilized thrombin binding aptamer. The selectivity of the sensor is demonstrated by the specific discrimination of thrombin from other protein analytes. The specific recognition and binding of thrombin by the thrombin binding aptamer is essential to the mechanism of the aptamer-based sensor, as shown through measurements using negative control oligonucleotides. In addition, the sensor can detect 1 nM thrombin in the presence of complex biofluids, such as 10% fetal calf serum, demonstrating that the immobilized, 5′-capped, 3′-capped aptamer is sufficiently robust for clinical diagnostic applications. Furthermore, the proposed sensor may be implemented for multiplexed detection using different aptamer-Raman probe complexes.

Original languageEnglish (US)
Pages (from-to)4386-4390
Number of pages5
JournalNano Letters
Volume8
Issue number12
DOIs
StatePublished - Dec 2008

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

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  • Cite this

    Cho, H., Baker, B. R., Wachsmann-Hogiu, S., Pagba, C. V., Laurence, T. A., Lane, S. M., Lee, L. P., & Tok, J. B. H. (2008). Aptamer-based SERRS sensor for thrombin detection. Nano Letters, 8(12), 4386-4390. https://doi.org/10.1021/nl802245w