Aptamer-based SERRS sensor for thrombin detection

Hansang Cho, Brian R. Baker, Sebastian Wachsmann-Hogiu, Cynthia V. Pagba, Ted A. Laurence, Stephen M. Lane, Luke P. Lee, Jeffrey B H Tok

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

We describe an aptamer-based surface enhanced resonance Raman scattering (SERRS) sensor with high sensitivity, specificity, and stability for the detection of a coagulation protein, human α-thrombin. The sensor achieves high sensitivity and a limit of detection of 100 pM by monitoring the SERRS signal change upon the single-step of thrombin binding to immobilized thrombin binding aptamer. The selectivity of the sensor is demonstrated by the specific discrimination of thrombin from other protein analytes. The specific recognition and binding of thrombin by the thrombin binding aptamer is essential to the mechanism of the aptamer-based sensor, as shown through measurements using negative control oligonucleotides. In addition, the sensor can detect 1 nM thrombin in the presence of complex biofluids, such as 10% fetal calf serum, demonstrating that the immobilized, 5′-capped, 3′-capped aptamer is sufficiently robust for clinical diagnostic applications. Furthermore, the proposed sensor may be implemented for multiplexed detection using different aptamer-Raman probe complexes.

Original languageEnglish (US)
Pages (from-to)4386-4390
Number of pages5
JournalNano Letters
Volume8
Issue number12
DOIs
StatePublished - Dec 2008

Fingerprint

thrombin
resonance scattering
Thrombin
Raman scattering
Raman spectra
sensors
Sensors
Proteins
proteins
Oligonucleotides
calves
Coagulation
oligonucleotides
sensitivity
coagulation
serums
discrimination
selectivity
Monitoring
probes

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Cho, H., Baker, B. R., Wachsmann-Hogiu, S., Pagba, C. V., Laurence, T. A., Lane, S. M., ... Tok, J. B. H. (2008). Aptamer-based SERRS sensor for thrombin detection. Nano Letters, 8(12), 4386-4390. https://doi.org/10.1021/nl802245w

Aptamer-based SERRS sensor for thrombin detection. / Cho, Hansang; Baker, Brian R.; Wachsmann-Hogiu, Sebastian; Pagba, Cynthia V.; Laurence, Ted A.; Lane, Stephen M.; Lee, Luke P.; Tok, Jeffrey B H.

In: Nano Letters, Vol. 8, No. 12, 12.2008, p. 4386-4390.

Research output: Contribution to journalArticle

Cho, H, Baker, BR, Wachsmann-Hogiu, S, Pagba, CV, Laurence, TA, Lane, SM, Lee, LP & Tok, JBH 2008, 'Aptamer-based SERRS sensor for thrombin detection', Nano Letters, vol. 8, no. 12, pp. 4386-4390. https://doi.org/10.1021/nl802245w
Cho H, Baker BR, Wachsmann-Hogiu S, Pagba CV, Laurence TA, Lane SM et al. Aptamer-based SERRS sensor for thrombin detection. Nano Letters. 2008 Dec;8(12):4386-4390. https://doi.org/10.1021/nl802245w
Cho, Hansang ; Baker, Brian R. ; Wachsmann-Hogiu, Sebastian ; Pagba, Cynthia V. ; Laurence, Ted A. ; Lane, Stephen M. ; Lee, Luke P. ; Tok, Jeffrey B H. / Aptamer-based SERRS sensor for thrombin detection. In: Nano Letters. 2008 ; Vol. 8, No. 12. pp. 4386-4390.
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