Application of the luciferase recombinant cell culture bioassay system for the analysis of polycyclic aromatic hydrocarbons

Michael H Ziccardi, Ian Gardner, Michael S. Denison

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

An aryl hydrocarbon (Ah) receptor-based luciferase cell culture bioassay developed to detect 2,3,7,8-tetrachlorodibenzop-dioxin (TCDD) and other halogenated aromatics was modified and optimized to detect and quantitate polycyclic aromatics (PAHs). Twenty-four PAHs were analyzed, and subsequent EC50 and EC20 concentrations (based on the median and 20% TCDD maximal response, respectively) and appropriate induction equivalency factors (calculated by comparison to the response obtained with TCDD) were determined from dose-response experiments. Six compounds were shown to be active in the system, with benzo[k]fluoranthene 〉 benz[a,h]anthracene ≈ indeno[1,2,3-cd]pyrene 〉 benzo[a]pyrene 〉 benzo[b]fluoranthene 〉 chrysene. A complex mixture of 16 PAHs was also analyzed using this system, and overall induction equivalency (or I-EQ) of the mixture was shown to be very similar to that predicted from the sum of the activity estimated for each individual PAH. Overall, our results strongly support the use of this system for the detection and relative quantitation of Ah receptor-active PAHs.

Original languageEnglish (US)
Pages (from-to)2027-2033
Number of pages7
JournalEnvironmental Toxicology and Chemistry
Volume21
Issue number10
StatePublished - Oct 2002

Fingerprint

Dioxins
Bioassay
Polycyclic Aromatic Hydrocarbons
Polycyclic aromatic hydrocarbons
Systems Analysis
Luciferases
Cell culture
Biological Assay
bioassay
Aryl Hydrocarbon Receptors
PAH
Cell Culture Techniques
dioxin
fluoranthene
pyrene
Benzo(a)pyrene
Complex Mixtures
hydrocarbon
analysis
experiment

Keywords

  • Aryl hydrocarbon receptor
  • Cytochrome P4501A1
  • Induction equivalency
  • Luciferase
  • Polycyclic aromatic hydrocarbon

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

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abstract = "An aryl hydrocarbon (Ah) receptor-based luciferase cell culture bioassay developed to detect 2,3,7,8-tetrachlorodibenzop-dioxin (TCDD) and other halogenated aromatics was modified and optimized to detect and quantitate polycyclic aromatics (PAHs). Twenty-four PAHs were analyzed, and subsequent EC50 and EC20 concentrations (based on the median and 20{\%} TCDD maximal response, respectively) and appropriate induction equivalency factors (calculated by comparison to the response obtained with TCDD) were determined from dose-response experiments. Six compounds were shown to be active in the system, with benzo[k]fluoranthene 〉 benz[a,h]anthracene ≈ indeno[1,2,3-cd]pyrene 〉 benzo[a]pyrene 〉 benzo[b]fluoranthene 〉 chrysene. A complex mixture of 16 PAHs was also analyzed using this system, and overall induction equivalency (or I-EQ) of the mixture was shown to be very similar to that predicted from the sum of the activity estimated for each individual PAH. Overall, our results strongly support the use of this system for the detection and relative quantitation of Ah receptor-active PAHs.",
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AU - Gardner, Ian

AU - Denison, Michael S.

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AB - An aryl hydrocarbon (Ah) receptor-based luciferase cell culture bioassay developed to detect 2,3,7,8-tetrachlorodibenzop-dioxin (TCDD) and other halogenated aromatics was modified and optimized to detect and quantitate polycyclic aromatics (PAHs). Twenty-four PAHs were analyzed, and subsequent EC50 and EC20 concentrations (based on the median and 20% TCDD maximal response, respectively) and appropriate induction equivalency factors (calculated by comparison to the response obtained with TCDD) were determined from dose-response experiments. Six compounds were shown to be active in the system, with benzo[k]fluoranthene 〉 benz[a,h]anthracene ≈ indeno[1,2,3-cd]pyrene 〉 benzo[a]pyrene 〉 benzo[b]fluoranthene 〉 chrysene. A complex mixture of 16 PAHs was also analyzed using this system, and overall induction equivalency (or I-EQ) of the mixture was shown to be very similar to that predicted from the sum of the activity estimated for each individual PAH. Overall, our results strongly support the use of this system for the detection and relative quantitation of Ah receptor-active PAHs.

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