Abstract
A synthetic chemical library comprised of alkylated and acylated amino acids was synthesized and screened to determine structures that bind to a model target, streptavidin. The library was prepared using 'split synthesis' and screened in a solid phase binding assay. The structure of positively reacting compounds was determined using mass spectroscopy. Positive compounds, together with various structural analogs were synthesized and their binding confirmed. Structures containing both an imidazole moiety and a substituted aromatic residue demonstrated binding.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 146-156 |
| Number of pages | 11 |
| Journal | Drug Development Research |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1994 |
| Externally published | Yes |
Fingerprint
Keywords
- Split synthesis
- Streptavidin
- Synthetic chemical library
ASJC Scopus subject areas
- Drug Discovery
- Organic Chemistry
- Pharmacology
Cite this
Application of one-read one-structure approach to identification of nonpeptidic ligands. / Stankova, M.; Issakova, O.; Sepetov, N. F.; Krchnak, V.; Lam, Kit; Lebl, M.
In: Drug Development Research, Vol. 33, No. 2, 1994, p. 146-156.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of one-read one-structure approach to identification of nonpeptidic ligands
AU - Stankova, M.
AU - Issakova, O.
AU - Sepetov, N. F.
AU - Krchnak, V.
AU - Lam, Kit
AU - Lebl, M.
PY - 1994
Y1 - 1994
N2 - A synthetic chemical library comprised of alkylated and acylated amino acids was synthesized and screened to determine structures that bind to a model target, streptavidin. The library was prepared using 'split synthesis' and screened in a solid phase binding assay. The structure of positively reacting compounds was determined using mass spectroscopy. Positive compounds, together with various structural analogs were synthesized and their binding confirmed. Structures containing both an imidazole moiety and a substituted aromatic residue demonstrated binding.
AB - A synthetic chemical library comprised of alkylated and acylated amino acids was synthesized and screened to determine structures that bind to a model target, streptavidin. The library was prepared using 'split synthesis' and screened in a solid phase binding assay. The structure of positively reacting compounds was determined using mass spectroscopy. Positive compounds, together with various structural analogs were synthesized and their binding confirmed. Structures containing both an imidazole moiety and a substituted aromatic residue demonstrated binding.
KW - Split synthesis
KW - Streptavidin
KW - Synthetic chemical library
UR - http://www.scopus.com/inward/record.url?scp=0028153337&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028153337&partnerID=8YFLogxK
U2 - 10.1002/ddr.430330211
DO - 10.1002/ddr.430330211
M3 - Article
AN - SCOPUS:0028153337
VL - 33
SP - 146
EP - 156
JO - Drug Development Research
JF - Drug Development Research
SN - 0272-4391
IS - 2
ER -