Abstract
The glycome, that is, the glycan components of a biological source, has been widely reported to change with disease states. However, mining the glycome for biomarkers is complicated by glycan structural heterogeneity. Nanoflow LC, or nano-LC, significantly addresses the problem by providing a highly sensitive and quantitative method of separating and profiling glycans. This review summarizes recent advances in analytical technology and methodology that enhance and augment the advantages offered by nano-LC. (e.g., reversed phase, hydrophilic interaction and porous graphitized carbon chromatography, as well as associated derivatization strategies), detectors (e.g., fluorescence and MS), and technology platforms (particularly chip-based nano-LC) are examined in detail, along with their application to biomarker discovery. Particular emphasis is placed on methods and technologies that allow structure-specific glycan profiling.
Original language | English (US) |
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Pages (from-to) | 2573-2585 |
Number of pages | 13 |
Journal | Bioanalysis |
Volume | 3 |
Issue number | 22 |
DOIs | |
State | Published - Nov 2011 |
ASJC Scopus subject areas
- Clinical Biochemistry
- Pharmacology, Toxicology and Pharmaceutics(all)
- Medical Laboratory Technology
- Analytical Chemistry