Application of multiplex quantitative Polymerase chain reaction methods to detect common bacterial fish pathogens in Nile tilapia, Oreochromis niloticus, hatcheries in Costa Rica

Adrián López-Porras, Carolina Elizondo, Aida J. Chaves, Alvin C. Camus, Matt J. Griffin, Kirsten Kenelty, Samantha Barum, Elías Barquero-Calvo, Esteban Soto Martinez

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Edwardsiella spp., Streptococcus spp., and Francisella noatunensis subsp. orientalis are some of the most important fish pathogens affecting global tilapia, Oreochromis spp., aquaculture. In Costa Rica, the aquaculture industry is dominated by freshwater-cultured Nile tilapia, Oreochromis niloticus, which are raised in all seven national provinces. At present, little is known regarding the diversity of pathogens present in these facilities, and definitive identification of agents associated with disease outbreaks are rare. To evaluate the prevalence of common bacterial pathogens in these systems, this study used multiplex quantitative polymerase chain reaction (qPCR) assays targeting Edwardsiella, Streptococcus, and Francisella species as a diagnostic and surveillance tool. In 2017, seven different tilapia hatcheries were visited, and 350 fingerlings were subjected to necropsy and molecular diagnostic evaluation. Fish exhibiting gross signs of disease were subjected to histological and microbiological analysis. For the first time, Edwardsiella anguillarum was recovered and molecularly confirmed from diseased tilapia in Costa Rica. In addition, F. noatunensis subsp. orientalis was identified in a region of Costa Rica where it had not been previously reported.

Original languageEnglish (US)
JournalJournal of the World Aquaculture Society
DOIs
StateAccepted/In press - Jan 1 2018

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Edwardsiella
Tilapia (Cichlidae)
Oreochromis niloticus
Francisella
hatchery
Costa Rica
polymerase chain reaction
hatcheries
quantitative polymerase chain reaction
pathogen
Streptococcus
pathogens
fish
Oreochromis
aquaculture industry
fingerlings
targeting
aquaculture
necropsy
methodology

Keywords

  • Costa Rica
  • diagnosis
  • pathogen
  • tilapia

ASJC Scopus subject areas

  • Aquatic Science
  • Agronomy and Crop Science

Cite this

Application of multiplex quantitative Polymerase chain reaction methods to detect common bacterial fish pathogens in Nile tilapia, Oreochromis niloticus, hatcheries in Costa Rica. / López-Porras, Adrián; Elizondo, Carolina; Chaves, Aida J.; Camus, Alvin C.; Griffin, Matt J.; Kenelty, Kirsten; Barum, Samantha; Barquero-Calvo, Elías; Soto Martinez, Esteban.

In: Journal of the World Aquaculture Society, 01.01.2018.

Research output: Contribution to journalArticle

López-Porras, Adrián ; Elizondo, Carolina ; Chaves, Aida J. ; Camus, Alvin C. ; Griffin, Matt J. ; Kenelty, Kirsten ; Barum, Samantha ; Barquero-Calvo, Elías ; Soto Martinez, Esteban. / Application of multiplex quantitative Polymerase chain reaction methods to detect common bacterial fish pathogens in Nile tilapia, Oreochromis niloticus, hatcheries in Costa Rica. In: Journal of the World Aquaculture Society. 2018.
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