Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan

M. E.H. Mohammed, I. E. Aradaib, M. M. Mukhtar, H. W. Ghalib, H. P. Riemann, A. Oyejide, Bennie Osburn

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Epizootic hemorrhagic disease virus (EHDV), isolate 318 (EHDV-318), an untyped virus recovered from a sentinel calf herd at the Khartoum University farm in central Sudan, was characterized using molecular biological techniques. With dot blot hybridization technique, a cDNA probe derived from genome segment 6 of EHDV-2 (Alberta strain) hybridized with RNA from EHDV-318. Application of serogroup-specific EHDV polymerase chain reaction (PCR) to EHDV-318 RNA resulted in specific amplification of a 387 bp PCR product. Amplification product was visualized on ethidium bromide-stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with a non-radiolabelled internal probe. No amplification product or hybridization signal was detected when the serotype-specific EHDV-1 or EHDV-2 PCR-based assays were applied to RNA from EHDV-318. The scientific data presented in this study indicated that cDNA probes and serogroup-specific PCR-based assay can de used to classify the virus as a member of EHDV serogroup, and as serotypically distinct from EHDV-1 and EHDV-2.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalVeterinary Microbiology
Volume52
Issue number3-4
DOIs
StatePublished - Oct 1 1996

Fingerprint

Epizootic hemorrhagic disease virus
Sudan
calves
polymerase chain reaction
serotypes
Polymerase Chain Reaction
methodology
RNA
cyhalothrin
hybridization
Complementary DNA
Viruses
viruses
Alberta
Ethidium
assays
nucleic acid hybridization
Sepharose
agarose

Keywords

  • Cattle-viruses
  • CDNA
  • Diagnosis-viruses
  • DNA probe
  • Epizootic hemorrhagic disease virus
  • Orbiviruses
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan. / Mohammed, M. E.H.; Aradaib, I. E.; Mukhtar, M. M.; Ghalib, H. W.; Riemann, H. P.; Oyejide, A.; Osburn, Bennie.

In: Veterinary Microbiology, Vol. 52, No. 3-4, 01.10.1996, p. 201-208.

Research output: Contribution to journalArticle

Mohammed, M. E.H. ; Aradaib, I. E. ; Mukhtar, M. M. ; Ghalib, H. W. ; Riemann, H. P. ; Oyejide, A. ; Osburn, Bennie. / Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan. In: Veterinary Microbiology. 1996 ; Vol. 52, No. 3-4. pp. 201-208.
@article{9115d33f31544a04a638bcbdebf0d504,
title = "Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan",
abstract = "Epizootic hemorrhagic disease virus (EHDV), isolate 318 (EHDV-318), an untyped virus recovered from a sentinel calf herd at the Khartoum University farm in central Sudan, was characterized using molecular biological techniques. With dot blot hybridization technique, a cDNA probe derived from genome segment 6 of EHDV-2 (Alberta strain) hybridized with RNA from EHDV-318. Application of serogroup-specific EHDV polymerase chain reaction (PCR) to EHDV-318 RNA resulted in specific amplification of a 387 bp PCR product. Amplification product was visualized on ethidium bromide-stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with a non-radiolabelled internal probe. No amplification product or hybridization signal was detected when the serotype-specific EHDV-1 or EHDV-2 PCR-based assays were applied to RNA from EHDV-318. The scientific data presented in this study indicated that cDNA probes and serogroup-specific PCR-based assay can de used to classify the virus as a member of EHDV serogroup, and as serotypically distinct from EHDV-1 and EHDV-2.",
keywords = "Cattle-viruses, CDNA, Diagnosis-viruses, DNA probe, Epizootic hemorrhagic disease virus, Orbiviruses, Polymerase chain reaction",
author = "Mohammed, {M. E.H.} and Aradaib, {I. E.} and Mukhtar, {M. M.} and Ghalib, {H. W.} and Riemann, {H. P.} and A. Oyejide and Bennie Osburn",
year = "1996",
month = "10",
day = "1",
doi = "10.1016/S0378-1135(96)00073-9",
language = "English (US)",
volume = "52",
pages = "201--208",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan

AU - Mohammed, M. E.H.

AU - Aradaib, I. E.

AU - Mukhtar, M. M.

AU - Ghalib, H. W.

AU - Riemann, H. P.

AU - Oyejide, A.

AU - Osburn, Bennie

PY - 1996/10/1

Y1 - 1996/10/1

N2 - Epizootic hemorrhagic disease virus (EHDV), isolate 318 (EHDV-318), an untyped virus recovered from a sentinel calf herd at the Khartoum University farm in central Sudan, was characterized using molecular biological techniques. With dot blot hybridization technique, a cDNA probe derived from genome segment 6 of EHDV-2 (Alberta strain) hybridized with RNA from EHDV-318. Application of serogroup-specific EHDV polymerase chain reaction (PCR) to EHDV-318 RNA resulted in specific amplification of a 387 bp PCR product. Amplification product was visualized on ethidium bromide-stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with a non-radiolabelled internal probe. No amplification product or hybridization signal was detected when the serotype-specific EHDV-1 or EHDV-2 PCR-based assays were applied to RNA from EHDV-318. The scientific data presented in this study indicated that cDNA probes and serogroup-specific PCR-based assay can de used to classify the virus as a member of EHDV serogroup, and as serotypically distinct from EHDV-1 and EHDV-2.

AB - Epizootic hemorrhagic disease virus (EHDV), isolate 318 (EHDV-318), an untyped virus recovered from a sentinel calf herd at the Khartoum University farm in central Sudan, was characterized using molecular biological techniques. With dot blot hybridization technique, a cDNA probe derived from genome segment 6 of EHDV-2 (Alberta strain) hybridized with RNA from EHDV-318. Application of serogroup-specific EHDV polymerase chain reaction (PCR) to EHDV-318 RNA resulted in specific amplification of a 387 bp PCR product. Amplification product was visualized on ethidium bromide-stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with a non-radiolabelled internal probe. No amplification product or hybridization signal was detected when the serotype-specific EHDV-1 or EHDV-2 PCR-based assays were applied to RNA from EHDV-318. The scientific data presented in this study indicated that cDNA probes and serogroup-specific PCR-based assay can de used to classify the virus as a member of EHDV serogroup, and as serotypically distinct from EHDV-1 and EHDV-2.

KW - Cattle-viruses

KW - CDNA

KW - Diagnosis-viruses

KW - DNA probe

KW - Epizootic hemorrhagic disease virus

KW - Orbiviruses

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0030271722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030271722&partnerID=8YFLogxK

U2 - 10.1016/S0378-1135(96)00073-9

DO - 10.1016/S0378-1135(96)00073-9

M3 - Article

VL - 52

SP - 201

EP - 208

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -