Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan

M. E.H. Mohammed, I. E. Aradaib, M. M. Mukhtar, H. W. Ghalib, H. P. Riemann, A. Oyejide, Bennie Osburn

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Epizootic hemorrhagic disease virus (EHDV), isolate 318 (EHDV-318), an untyped virus recovered from a sentinel calf herd at the Khartoum University farm in central Sudan, was characterized using molecular biological techniques. With dot blot hybridization technique, a cDNA probe derived from genome segment 6 of EHDV-2 (Alberta strain) hybridized with RNA from EHDV-318. Application of serogroup-specific EHDV polymerase chain reaction (PCR) to EHDV-318 RNA resulted in specific amplification of a 387 bp PCR product. Amplification product was visualized on ethidium bromide-stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with a non-radiolabelled internal probe. No amplification product or hybridization signal was detected when the serotype-specific EHDV-1 or EHDV-2 PCR-based assays were applied to RNA from EHDV-318. The scientific data presented in this study indicated that cDNA probes and serogroup-specific PCR-based assay can de used to classify the virus as a member of EHDV serogroup, and as serotypically distinct from EHDV-1 and EHDV-2.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalVeterinary Microbiology
Volume52
Issue number3-4
DOIs
StatePublished - Oct 1 1996

Keywords

  • Cattle-viruses
  • CDNA
  • Diagnosis-viruses
  • DNA probe
  • Epizootic hemorrhagic disease virus
  • Orbiviruses
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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