Application of modular kinetic analysis to mitochondrial oxidative phosphorylation in skeletal muscle of birds exposed to acute heat stress

Motoi Kikusato, Jon J Ramsey, Taku Amo, Masaaki Toyomizu

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (ΔΨ). To clarify the mechanism underlying the elevation of ΔΨ, modular kinetic analysis was applied to oxidative phosphorylation in skeletal muscle mitochondria of heat-stressed birds (34°C for 12. h). In the birds exposed to heat stress, 'substrate oxidation' (a ΔΨ-producer) was increased compared to control (24°C) birds, although there was little difference in 'proton leak' (a ΔΨ-consumer), suggesting that an elevation in the ΔΨ at state 4 may be due to enhanced substrate oxidation. It thus appears that enhanced substrate oxidation plays a crucial role in the overproduction of ROS for heat-stressed birds, probably via elevated ΔΨ.

Original languageEnglish (US)
Pages (from-to)3143-3148
Number of pages6
JournalFEBS Letters
Volume584
Issue number14
DOIs
StatePublished - Jul 2010

Keywords

  • Coupling efficiency
  • Heat stress
  • Membrane potential (ΔΨ)
  • Modular kinetic analysis
  • Reactive oxygen species
  • Substrate oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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