Abstract
The applicability of matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry to the analysis of porphyrins has been examined. High resolution spectra were readily obtained with good sensitivity and a detection limit as low as 2 fmol. A mixed solvent system of toluene and ethanol (1:1 by volume) proved to be compatible with the 2,5-dihydroxybenzoic acid (DHB) matrix and solubilized most of the porphyrins examined in this study. Porphyrins which were insoluble in this solvent mixture could be dissolved in an appropriate solvent and deposited on a layer of DHB (layered MALDI). The parent ion was generally the largest peak in the spectrum, although for some metalloporphyrins the peak corresponding to (M - metal + 3H)+ was dominant. The extent of demetallation was found to depend on factors such as sample preparation, the metal ion and the laser intensity. Addition of more than one hydrogen occurred in many cases but was a minor process. Spectra of compounds ionized by different methods showed increasing fragmentation in the order MALDI <layered MALDI < fast-atom bombardment <laser desorption.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 439-451 |
| Number of pages | 13 |
| Journal | European Journal of Mass Spectrometry |
| Volume | 3 |
| Issue number | 6 |
| State | Published - 1997 |
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Keywords
- Fast atom bombardment
- Fourier transform mass spectrometry
- Laser desorption
- Matrix-assisted laser desorption/ionization
- Porphyrins
ASJC Scopus subject areas
- Spectroscopy
- Atomic and Molecular Physics, and Optics
Cite this
Application of matrix-assisted laser desorption/ionization Fourier transform mass spectrometry to the analysis of planar porphyrins and highly substituted nonplanar porphyrins. / Green, M. Kirk; Medforth, Craig J.; Muzzi, Cinzia M.; Nurco, Daniel J.; Shea, Kalyn M.; Smith, Kevin M.; Lebrilla, Carlito B; Shelnutt, John A.
In: European Journal of Mass Spectrometry, Vol. 3, No. 6, 1997, p. 439-451.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of matrix-assisted laser desorption/ionization Fourier transform mass spectrometry to the analysis of planar porphyrins and highly substituted nonplanar porphyrins
AU - Green, M. Kirk
AU - Medforth, Craig J.
AU - Muzzi, Cinzia M.
AU - Nurco, Daniel J.
AU - Shea, Kalyn M.
AU - Smith, Kevin M.
AU - Lebrilla, Carlito B
AU - Shelnutt, John A.
PY - 1997
Y1 - 1997
N2 - The applicability of matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry to the analysis of porphyrins has been examined. High resolution spectra were readily obtained with good sensitivity and a detection limit as low as 2 fmol. A mixed solvent system of toluene and ethanol (1:1 by volume) proved to be compatible with the 2,5-dihydroxybenzoic acid (DHB) matrix and solubilized most of the porphyrins examined in this study. Porphyrins which were insoluble in this solvent mixture could be dissolved in an appropriate solvent and deposited on a layer of DHB (layered MALDI). The parent ion was generally the largest peak in the spectrum, although for some metalloporphyrins the peak corresponding to (M - metal + 3H)+ was dominant. The extent of demetallation was found to depend on factors such as sample preparation, the metal ion and the laser intensity. Addition of more than one hydrogen occurred in many cases but was a minor process. Spectra of compounds ionized by different methods showed increasing fragmentation in the order MALDI
AB - The applicability of matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry to the analysis of porphyrins has been examined. High resolution spectra were readily obtained with good sensitivity and a detection limit as low as 2 fmol. A mixed solvent system of toluene and ethanol (1:1 by volume) proved to be compatible with the 2,5-dihydroxybenzoic acid (DHB) matrix and solubilized most of the porphyrins examined in this study. Porphyrins which were insoluble in this solvent mixture could be dissolved in an appropriate solvent and deposited on a layer of DHB (layered MALDI). The parent ion was generally the largest peak in the spectrum, although for some metalloporphyrins the peak corresponding to (M - metal + 3H)+ was dominant. The extent of demetallation was found to depend on factors such as sample preparation, the metal ion and the laser intensity. Addition of more than one hydrogen occurred in many cases but was a minor process. Spectra of compounds ionized by different methods showed increasing fragmentation in the order MALDI
KW - Fast atom bombardment
KW - Fourier transform mass spectrometry
KW - Laser desorption
KW - Matrix-assisted laser desorption/ionization
KW - Porphyrins
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UR - http://www.scopus.com/inward/citedby.url?scp=0001211895&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0001211895
VL - 3
SP - 439
EP - 451
JO - European Journal of Mass Spectrometry
JF - European Journal of Mass Spectrometry
SN - 1469-0667
IS - 6
ER -