Application of laser tweezers Raman spectroscopy techniques to the monitoring of single cell response to stimuli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Laser tweezers Raman spectroscopy (LTRS) combines optical trapping with micro-Raman spectroscopy to enable label-free biochemical analysis of individual cells and small biological particles in suspension. The integration of the two technologies greatly simplifies the sample preparation and handling of suspension cells for spectroscopic analysis in physiologically meaningful conditions. In our group, LTRS has been used to study the effects of external perturbations, both chemical and mechanical, on the biochemistry of the cell. Single cell dynamics can be studied by performing longitudinal studies to continuously monitor the response of the cell as it interacts with its environment. The ability to carry out these measurements in-vitro makes LTRS an attractive tool for many biomedical applications. Here, we discuss the use of LTRS to study the response of cancer cells to chemotherapeutics and bacteria cells to antibiotics and show that the life cycle and apoptosis of the cells can be detected. These results show the promise of LTRS for drug discovery/screening, antibiotic susceptibility testing, and chemotherapy response monitoring applications. In separate experiments, we study the response of red blood cells to the mechanical forces imposed on the cell by the optical tweezers. A laser power dependent deoxygenation of the red blood cell in the single beam trap is reported. Normal, sickle cell, and fetal red blood cells have a different behavior that enables the discrimination of the cell types based on this mechanochemical response. These results show the potential utility of LTRS for diagnosing and studying red blood cell diseases.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8427
DOIs
StatePublished - 2012
EventBiophotonics: Photonic Solutions for Better Health Care III - Brussels, Belgium
Duration: Apr 16 2012Apr 19 2012

Other

OtherBiophotonics: Photonic Solutions for Better Health Care III
CountryBelgium
CityBrussels
Period4/16/124/19/12

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Keywords

  • Chemotherapy
  • Deoxygenation
  • Laser tweezers
  • Leukemia
  • Optical trapping
  • Raman spectroscopy
  • Red blood cells
  • Sickle cell

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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