Application of high-content cell imaging system in cell proliferation detection

Li Wu, Yu Tai, Shan Shan Hu, Rui Wang, Mei Zhang, Juan Tao, Wei Jie Zhou, Qingtong Wang, Wei Wei

Research output: Contribution to journalArticle

Abstract

Aim To compare high-content cell imaging system and other methods in detecting cell proliferation, including the traditional thymidine (3H-TdR) incorporation method, methyl thiazolyl tetrazolium (MTT) method and cell counting kit-8 (CCK-8) method. Methods The fibroblast-like synovial cells (FLS) were used as the study object to observe the sensitivity and stability of FLS proliferation in different methods by using the usual proliferative stimulant tumor necrosis factor-a (TNF-a) and the known proliferation inhibitor methotrexate at different concentrations. Results The 3H-TdR method and high-content cell imaging could detect a significant inhibitory effect of 1 nmol L"1 MTX on FLS cell proliferation, MTT assay and CCK-8 method could detect the significant inhibitory effect of 10 nmol L"1 MTX on FLS cell proliferation. 3H-TdR method was found to have a large degree of discretization in the data set, with a standard deviation of 32. 61%-61. 36%, and the MTT method was 11.9%-17.8%, the CCK-8 method was 17.15%-32. 88%, and the high-content cell imaging system method was 12.66%-26.54%. Conclusion The method of high-content cell imaging system is more accurate and stable for detecting cell proliferation.

Original languageEnglish (US)
Pages (from-to)1024-1029
Number of pages6
JournalChinese Pharmacological Bulletin
Volume34
Issue number7
DOIs
StatePublished - Jul 1 2018
Externally publishedYes

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Cell Proliferation
Fibroblasts
Methotrexate
Thymidine
Tumor Necrosis Factor-alpha

Keywords

  • 3H-TdR participation method
  • CCK-8
  • Cell proliferation
  • Fibroblast-like synoviocytes
  • High-content cell imaging system
  • Methotrexate
  • MTT
  • TNF-α

ASJC Scopus subject areas

  • Pharmacology

Cite this

Application of high-content cell imaging system in cell proliferation detection. / Wu, Li; Tai, Yu; Hu, Shan Shan; Wang, Rui; Zhang, Mei; Tao, Juan; Zhou, Wei Jie; Wang, Qingtong; Wei, Wei.

In: Chinese Pharmacological Bulletin, Vol. 34, No. 7, 01.07.2018, p. 1024-1029.

Research output: Contribution to journalArticle

Wu, L, Tai, Y, Hu, SS, Wang, R, Zhang, M, Tao, J, Zhou, WJ, Wang, Q & Wei, W 2018, 'Application of high-content cell imaging system in cell proliferation detection', Chinese Pharmacological Bulletin, vol. 34, no. 7, pp. 1024-1029. https://doi.org/10.3969/i.issn.1001-1978.2018.07.025
Wu, Li ; Tai, Yu ; Hu, Shan Shan ; Wang, Rui ; Zhang, Mei ; Tao, Juan ; Zhou, Wei Jie ; Wang, Qingtong ; Wei, Wei. / Application of high-content cell imaging system in cell proliferation detection. In: Chinese Pharmacological Bulletin. 2018 ; Vol. 34, No. 7. pp. 1024-1029.
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AU - Zhou, Wei Jie

AU - Wang, Qingtong

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AB - Aim To compare high-content cell imaging system and other methods in detecting cell proliferation, including the traditional thymidine (3H-TdR) incorporation method, methyl thiazolyl tetrazolium (MTT) method and cell counting kit-8 (CCK-8) method. Methods The fibroblast-like synovial cells (FLS) were used as the study object to observe the sensitivity and stability of FLS proliferation in different methods by using the usual proliferative stimulant tumor necrosis factor-a (TNF-a) and the known proliferation inhibitor methotrexate at different concentrations. Results The 3H-TdR method and high-content cell imaging could detect a significant inhibitory effect of 1 nmol L"1 MTX on FLS cell proliferation, MTT assay and CCK-8 method could detect the significant inhibitory effect of 10 nmol L"1 MTX on FLS cell proliferation. 3H-TdR method was found to have a large degree of discretization in the data set, with a standard deviation of 32. 61%-61. 36%, and the MTT method was 11.9%-17.8%, the CCK-8 method was 17.15%-32. 88%, and the high-content cell imaging system method was 12.66%-26.54%. Conclusion The method of high-content cell imaging system is more accurate and stable for detecting cell proliferation.

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