Application of europium(III) chelate-dyed nanoparticle labels in a competitive atrazine fluoroimmunoassay on an ITO waveguide

C. M. Cummins, M. E. Koivunen, A. Stephanian, S. J. Gee, B. D. Hammock, I. M. Kennedy

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We have demonstrated the use of an optical indium tin oxide (ITO) (quartz) waveguide as a new platform for immunosensors with fluorescent europium(III) chelate nanoparticle labels (Seradyn) in a competitive atrazine immunoassay. ITO as a solid surface facilitated the successful use of particulate labels in a competitive assay format. The limit of detection in the new nanoparticle assay was similar to a conventional ELISA. The effect of particle size on bioconjugate binding kinetics was studied using three sizes of bioconjugated particle labels (107, 304, and 396 nm) and a rabbit IgG/anti-IgG system in a 96-well plate. A decrease in particle size resulted in faster binding but did not increase the assay sensitivity. Flux calculations based on the particle diffusivity prove that faster binding of the small particles in this study was primarily due to diffusion kinetics and not necessarily to a higher density of antibodies on the particle surface. The results suggest that ITO could make a good platform for an optical immunosensor using fluorescent nanoparticle labels in a competitive assay format for small molecule detection. However, when used in combination with fluorescent particulate labels, a highly sensitive excitation/detection system needs to be developed to fully utilize the kinetic advantage from small particle size. Different regeneration methods tested in this study showed that repeated washings with 0.1 M glycine-HCl facilitated the reuse of the ITO waveguide.

Original languageEnglish (US)
Pages (from-to)1077-1085
Number of pages9
JournalBiosensors and Bioelectronics
Volume21
Issue number7
DOIs
StatePublished - Jan 15 2006

Fingerprint

Fluoroimmunoassay
Atrazine
Europium
Herbicides
Tin oxides
Particle Size
Nanoparticles
Indium
Labels
Waveguides
Assays
Immunosensors
Particle size
Kinetics
Quartz
Immunoassay
Glycine
Limit of Detection
Regeneration
Antibodies

Keywords

  • Atrazine
  • Fluorescence
  • Immunoassay
  • ITO
  • Lanthanide
  • Nanoparticles

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Application of europium(III) chelate-dyed nanoparticle labels in a competitive atrazine fluoroimmunoassay on an ITO waveguide. / Cummins, C. M.; Koivunen, M. E.; Stephanian, A.; Gee, S. J.; Hammock, B. D.; Kennedy, I. M.

In: Biosensors and Bioelectronics, Vol. 21, No. 7, 15.01.2006, p. 1077-1085.

Research output: Contribution to journalArticle

Cummins, C. M. ; Koivunen, M. E. ; Stephanian, A. ; Gee, S. J. ; Hammock, B. D. ; Kennedy, I. M. / Application of europium(III) chelate-dyed nanoparticle labels in a competitive atrazine fluoroimmunoassay on an ITO waveguide. In: Biosensors and Bioelectronics. 2006 ; Vol. 21, No. 7. pp. 1077-1085.
@article{81d74995812b462dac2429f5c8227ac9,
title = "Application of europium(III) chelate-dyed nanoparticle labels in a competitive atrazine fluoroimmunoassay on an ITO waveguide",
abstract = "We have demonstrated the use of an optical indium tin oxide (ITO) (quartz) waveguide as a new platform for immunosensors with fluorescent europium(III) chelate nanoparticle labels (Seradyn) in a competitive atrazine immunoassay. ITO as a solid surface facilitated the successful use of particulate labels in a competitive assay format. The limit of detection in the new nanoparticle assay was similar to a conventional ELISA. The effect of particle size on bioconjugate binding kinetics was studied using three sizes of bioconjugated particle labels (107, 304, and 396 nm) and a rabbit IgG/anti-IgG system in a 96-well plate. A decrease in particle size resulted in faster binding but did not increase the assay sensitivity. Flux calculations based on the particle diffusivity prove that faster binding of the small particles in this study was primarily due to diffusion kinetics and not necessarily to a higher density of antibodies on the particle surface. The results suggest that ITO could make a good platform for an optical immunosensor using fluorescent nanoparticle labels in a competitive assay format for small molecule detection. However, when used in combination with fluorescent particulate labels, a highly sensitive excitation/detection system needs to be developed to fully utilize the kinetic advantage from small particle size. Different regeneration methods tested in this study showed that repeated washings with 0.1 M glycine-HCl facilitated the reuse of the ITO waveguide.",
keywords = "Atrazine, Fluorescence, Immunoassay, ITO, Lanthanide, Nanoparticles",
author = "Cummins, {C. M.} and Koivunen, {M. E.} and A. Stephanian and Gee, {S. J.} and Hammock, {B. D.} and Kennedy, {I. M.}",
year = "2006",
month = "1",
day = "15",
doi = "10.1016/j.bios.2005.04.003",
language = "English (US)",
volume = "21",
pages = "1077--1085",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "7",

}

TY - JOUR

T1 - Application of europium(III) chelate-dyed nanoparticle labels in a competitive atrazine fluoroimmunoassay on an ITO waveguide

AU - Cummins, C. M.

AU - Koivunen, M. E.

AU - Stephanian, A.

AU - Gee, S. J.

AU - Hammock, B. D.

AU - Kennedy, I. M.

PY - 2006/1/15

Y1 - 2006/1/15

N2 - We have demonstrated the use of an optical indium tin oxide (ITO) (quartz) waveguide as a new platform for immunosensors with fluorescent europium(III) chelate nanoparticle labels (Seradyn) in a competitive atrazine immunoassay. ITO as a solid surface facilitated the successful use of particulate labels in a competitive assay format. The limit of detection in the new nanoparticle assay was similar to a conventional ELISA. The effect of particle size on bioconjugate binding kinetics was studied using three sizes of bioconjugated particle labels (107, 304, and 396 nm) and a rabbit IgG/anti-IgG system in a 96-well plate. A decrease in particle size resulted in faster binding but did not increase the assay sensitivity. Flux calculations based on the particle diffusivity prove that faster binding of the small particles in this study was primarily due to diffusion kinetics and not necessarily to a higher density of antibodies on the particle surface. The results suggest that ITO could make a good platform for an optical immunosensor using fluorescent nanoparticle labels in a competitive assay format for small molecule detection. However, when used in combination with fluorescent particulate labels, a highly sensitive excitation/detection system needs to be developed to fully utilize the kinetic advantage from small particle size. Different regeneration methods tested in this study showed that repeated washings with 0.1 M glycine-HCl facilitated the reuse of the ITO waveguide.

AB - We have demonstrated the use of an optical indium tin oxide (ITO) (quartz) waveguide as a new platform for immunosensors with fluorescent europium(III) chelate nanoparticle labels (Seradyn) in a competitive atrazine immunoassay. ITO as a solid surface facilitated the successful use of particulate labels in a competitive assay format. The limit of detection in the new nanoparticle assay was similar to a conventional ELISA. The effect of particle size on bioconjugate binding kinetics was studied using three sizes of bioconjugated particle labels (107, 304, and 396 nm) and a rabbit IgG/anti-IgG system in a 96-well plate. A decrease in particle size resulted in faster binding but did not increase the assay sensitivity. Flux calculations based on the particle diffusivity prove that faster binding of the small particles in this study was primarily due to diffusion kinetics and not necessarily to a higher density of antibodies on the particle surface. The results suggest that ITO could make a good platform for an optical immunosensor using fluorescent nanoparticle labels in a competitive assay format for small molecule detection. However, when used in combination with fluorescent particulate labels, a highly sensitive excitation/detection system needs to be developed to fully utilize the kinetic advantage from small particle size. Different regeneration methods tested in this study showed that repeated washings with 0.1 M glycine-HCl facilitated the reuse of the ITO waveguide.

KW - Atrazine

KW - Fluorescence

KW - Immunoassay

KW - ITO

KW - Lanthanide

KW - Nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=29144501316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29144501316&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2005.04.003

DO - 10.1016/j.bios.2005.04.003

M3 - Article

C2 - 16368482

AN - SCOPUS:29144501316

VL - 21

SP - 1077

EP - 1085

JO - Biosensors

JF - Biosensors

SN - 0956-5663

IS - 7

ER -