TY - JOUR
T1 - Application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs
AU - Jaing, Crystal J.
AU - Thissen, James B.
AU - Gardner, Shea N.
AU - McLoughlin, Kevin S.
AU - Hullinger, Pam
AU - Monday, Nicholas A.
AU - Niederwerder, Megan C.
AU - Rowland, Raymond R R
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Many of the disease syndromes challenging the commercial swine industry involve the analysis of complex problems caused by polymicrobial, emerging or reemerging, and transboundary pathogens. This study investigated the utility of the Lawrence Livermore Microbial Detection Array (Lawrence Livermore National Laboratory, Livermore, California), designed to detect 8,101 species of microbes, in the evaluation of known and unknown microbes in serum, oral fluid, and tonsil from pigs experimentally coinfected with Porcine reproductive and respiratory syndrome virus (PRRSV) and Porcine circovirus-2 (PCV-2). The array easily identified PRRSV and PCV-2, but at decreased sensitivities compared to standard polymerase chain reaction detection methods. The oral fluid sample was the most informative, possessing additional signatures for several swine-associated bacteria, including Streptococcus sp., Clostridium sp., and Staphylococcus sp.
AB - Many of the disease syndromes challenging the commercial swine industry involve the analysis of complex problems caused by polymicrobial, emerging or reemerging, and transboundary pathogens. This study investigated the utility of the Lawrence Livermore Microbial Detection Array (Lawrence Livermore National Laboratory, Livermore, California), designed to detect 8,101 species of microbes, in the evaluation of known and unknown microbes in serum, oral fluid, and tonsil from pigs experimentally coinfected with Porcine reproductive and respiratory syndrome virus (PRRSV) and Porcine circovirus-2 (PCV-2). The array easily identified PRRSV and PCV-2, but at decreased sensitivities compared to standard polymerase chain reaction detection methods. The oral fluid sample was the most informative, possessing additional signatures for several swine-associated bacteria, including Streptococcus sp., Clostridium sp., and Staphylococcus sp.
KW - Bacteria
KW - diagnostics
KW - disease
KW - microarray
KW - microbial
KW - pathogen
KW - pigs
KW - virus
UR - http://www.scopus.com/inward/record.url?scp=84930513029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930513029&partnerID=8YFLogxK
U2 - 10.1177/1040638715578484
DO - 10.1177/1040638715578484
M3 - Article
C2 - 25855363
AN - SCOPUS:84930513029
VL - 27
SP - 313
EP - 325
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 3
ER -