Application of a dual color detection scheme in the screening of a random combinatorial peptide library

Kit Lam, Shelly Wade, Farid Abdul-Latif, Michal Lebl

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Selectide Technology is a random synthetic combinatorial library method in which millions of random compounds are screened in parallel for their ability to bind to a tagged macromolecular target. The library consists of millions of beads and each individual bead expresses a unique chemical compound such as a peptide. In the standard enzyme-linked colorimetric detection scheme, the positive bead which turns color is isolated for microsequencing. In this paper, a dual color detection scheme using two sequential orthogonal probes is described. This dual color system enables one to rapidly differentiate false positive beads from true positive beads, resulting in a much more efficient use of the microsequencer.

Original languageEnglish (US)
Pages (from-to)219-223
Number of pages5
JournalJournal of Immunological Methods
Volume180
Issue number2
StatePublished - 1995
Externally publishedYes

Keywords

  • (Color)
  • Combinatorial library
  • Peptide
  • Screening
  • Selectide
  • Substrate

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biotechnology

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