Application of a dual color detection scheme in the screening of a random combinatorial peptide library

Kit Lam, Shelly Wade, Farid Abdul-Latif, Michal Lebl

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Selectide Technology is a random synthetic combinatorial library method in which millions of random compounds are screened in parallel for their ability to bind to a tagged macromolecular target. The library consists of millions of beads and each individual bead expresses a unique chemical compound such as a peptide. In the standard enzyme-linked colorimetric detection scheme, the positive bead which turns color is isolated for microsequencing. In this paper, a dual color detection scheme using two sequential orthogonal probes is described. This dual color system enables one to rapidly differentiate false positive beads from true positive beads, resulting in a much more efficient use of the microsequencer.

Original languageEnglish (US)
Pages (from-to)219-223
Number of pages5
JournalJournal of Immunological Methods
Volume180
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Peptide Library
Color
Libraries
Technology
Peptides
Enzymes

Keywords

  • (Color)
  • Combinatorial library
  • Peptide
  • Screening
  • Selectide
  • Substrate

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biotechnology

Cite this

Application of a dual color detection scheme in the screening of a random combinatorial peptide library. / Lam, Kit; Wade, Shelly; Abdul-Latif, Farid; Lebl, Michal.

In: Journal of Immunological Methods, Vol. 180, No. 2, 1995, p. 219-223.

Research output: Contribution to journalArticle

Lam, Kit ; Wade, Shelly ; Abdul-Latif, Farid ; Lebl, Michal. / Application of a dual color detection scheme in the screening of a random combinatorial peptide library. In: Journal of Immunological Methods. 1995 ; Vol. 180, No. 2. pp. 219-223.
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