Appearance of fibronectin during the differentiation of cartilage, bone, and bone marrow

R. E. Weiss, A Hari Reddi

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Fibronectin has been localized by indirect immunofluorescence during the various phases of endochondral bone formation in response to subcutaneously implanted demineralized bone matrix. Its histologic appearance has been correlated with results of biosynthetic experiments. The implanted collagenous bone matrix was coated with fibronectin before and during mesenchymal cell proliferation. During proliferation of mesenchymal precursor cells, the newly synthesized extracellular matrix exhibited a fibrillar network of fibronectin. During cartilage differentiation, the fibronectin in the extracellular matrix was apparently masked by proteoglycans, as judged by hyaluronidase treatment. Differentiating chondrocytes exhibited a uniform distribution of fibronectin. Fibronectin was present in a cottony array around osteoblasts during osteogenesis. The developing hematopoietic colonies revealed fibronectin associated with them. Therefore, it appears that fibronectin is ubiquitous throughout the development of endochondral bone and bone marrow.

Original languageEnglish (US)
Pages (from-to)630-636
Number of pages7
JournalJournal of Cell Biology
Volume88
Issue number3
StatePublished - 1981
Externally publishedYes

Fingerprint

Fibronectins
Cartilage
Bone Marrow
Bone and Bones
Bone Matrix
Osteogenesis
Extracellular Matrix
Hyaluronoglucosaminidase
Proteoglycans
Indirect Fluorescent Antibody Technique
Chondrocytes
Osteoblasts
Cell Proliferation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Appearance of fibronectin during the differentiation of cartilage, bone, and bone marrow. / Weiss, R. E.; Reddi, A Hari.

In: Journal of Cell Biology, Vol. 88, No. 3, 1981, p. 630-636.

Research output: Contribution to journalArticle

@article{62b66a911fad4d7aaf1d2d49bf7e4fa3,
title = "Appearance of fibronectin during the differentiation of cartilage, bone, and bone marrow",
abstract = "Fibronectin has been localized by indirect immunofluorescence during the various phases of endochondral bone formation in response to subcutaneously implanted demineralized bone matrix. Its histologic appearance has been correlated with results of biosynthetic experiments. The implanted collagenous bone matrix was coated with fibronectin before and during mesenchymal cell proliferation. During proliferation of mesenchymal precursor cells, the newly synthesized extracellular matrix exhibited a fibrillar network of fibronectin. During cartilage differentiation, the fibronectin in the extracellular matrix was apparently masked by proteoglycans, as judged by hyaluronidase treatment. Differentiating chondrocytes exhibited a uniform distribution of fibronectin. Fibronectin was present in a cottony array around osteoblasts during osteogenesis. The developing hematopoietic colonies revealed fibronectin associated with them. Therefore, it appears that fibronectin is ubiquitous throughout the development of endochondral bone and bone marrow.",
author = "Weiss, {R. E.} and Reddi, {A Hari}",
year = "1981",
language = "English (US)",
volume = "88",
pages = "630--636",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "3",

}

TY - JOUR

T1 - Appearance of fibronectin during the differentiation of cartilage, bone, and bone marrow

AU - Weiss, R. E.

AU - Reddi, A Hari

PY - 1981

Y1 - 1981

N2 - Fibronectin has been localized by indirect immunofluorescence during the various phases of endochondral bone formation in response to subcutaneously implanted demineralized bone matrix. Its histologic appearance has been correlated with results of biosynthetic experiments. The implanted collagenous bone matrix was coated with fibronectin before and during mesenchymal cell proliferation. During proliferation of mesenchymal precursor cells, the newly synthesized extracellular matrix exhibited a fibrillar network of fibronectin. During cartilage differentiation, the fibronectin in the extracellular matrix was apparently masked by proteoglycans, as judged by hyaluronidase treatment. Differentiating chondrocytes exhibited a uniform distribution of fibronectin. Fibronectin was present in a cottony array around osteoblasts during osteogenesis. The developing hematopoietic colonies revealed fibronectin associated with them. Therefore, it appears that fibronectin is ubiquitous throughout the development of endochondral bone and bone marrow.

AB - Fibronectin has been localized by indirect immunofluorescence during the various phases of endochondral bone formation in response to subcutaneously implanted demineralized bone matrix. Its histologic appearance has been correlated with results of biosynthetic experiments. The implanted collagenous bone matrix was coated with fibronectin before and during mesenchymal cell proliferation. During proliferation of mesenchymal precursor cells, the newly synthesized extracellular matrix exhibited a fibrillar network of fibronectin. During cartilage differentiation, the fibronectin in the extracellular matrix was apparently masked by proteoglycans, as judged by hyaluronidase treatment. Differentiating chondrocytes exhibited a uniform distribution of fibronectin. Fibronectin was present in a cottony array around osteoblasts during osteogenesis. The developing hematopoietic colonies revealed fibronectin associated with them. Therefore, it appears that fibronectin is ubiquitous throughout the development of endochondral bone and bone marrow.

UR - http://www.scopus.com/inward/record.url?scp=0019870937&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019870937&partnerID=8YFLogxK

M3 - Article

C2 - 7217207

AN - SCOPUS:0019870937

VL - 88

SP - 630

EP - 636

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 3

ER -