Apparently irreversible GTP hydrolysis attends tubulin self-assembly

James M Angelastro, D. L. Purich

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The pathway of GTP hydrolysis associated with microtubule polymerization was investigated using an assay of intermediate 18O-exchange reactions. Under a variety of conditions influencing tubulin self-assembly, GTP was hydrolyzed without any evidence of multiple reversals characteristic of reversible phosphoanhydride-bond cleavage. These results also accord with published findings that ATP hydrolysis during actin polymerization fails to display intermediate exchange reactions [Carlier, M.F., Pantaloni, D., Evans, J.A., Lambooy, P.K., Korn, E.D. and Webb, M.R. (1988) FEBS Lett. 235, 211-214].

Original languageEnglish (US)
Pages (from-to)507-511
Number of pages5
JournalEuropean Journal of Biochemistry
Issue number2
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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