Abstract
Intestinal colonization with toxigenic strains of Clostridium difficile was documented in 9 of 10 horses with acute onset diarrhea in a veterinary medical teaching hospital, whereas a similar isolate was detected in only 1 of 23 other horses without diarrhea in the hospital. One horse with diarrhea was infected simultaneously with both C. difficile and Salmonella krefeld. Clostridium difficile was detected by fecal culture on selective medium, confirmed with a latex particle agglutination test, and identified as toxigenic by polymerase chain reaction amplification of toxin A and toxin B gene sequences. Using an arbitrarily-primed polymerase chain reaction, 6 distinct C. difficile isolates were detected in the feces of the 9 affected horses at the time of the outbreak of diarrhea.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 343-346 |
| Number of pages | 4 |
| Journal | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc |
| Volume | 7 |
| Issue number | 3 |
| State | Published - Jul 1995 |
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ASJC Scopus subject areas
- Microbiology
- veterinary(all)
Cite this
Apparent outbreaks of Clostridium difficile-associated diarrhea in horses in a veterinary medical teaching hospital. / Madewell, B. R.; Tang, Y. J.; Madigan, John E; Madigan, J. E.; Hirsh, D. C.; Gumerlock, P. H.; Silva, J.
In: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Vol. 7, No. 3, 07.1995, p. 343-346.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apparent outbreaks of Clostridium difficile-associated diarrhea in horses in a veterinary medical teaching hospital.
AU - Madewell, B. R.
AU - Tang, Y. J.
AU - Madigan, John E
AU - Madigan, J. E.
AU - Hirsh, D. C.
AU - Gumerlock, P. H.
AU - Silva, J.
PY - 1995/7
Y1 - 1995/7
N2 - Intestinal colonization with toxigenic strains of Clostridium difficile was documented in 9 of 10 horses with acute onset diarrhea in a veterinary medical teaching hospital, whereas a similar isolate was detected in only 1 of 23 other horses without diarrhea in the hospital. One horse with diarrhea was infected simultaneously with both C. difficile and Salmonella krefeld. Clostridium difficile was detected by fecal culture on selective medium, confirmed with a latex particle agglutination test, and identified as toxigenic by polymerase chain reaction amplification of toxin A and toxin B gene sequences. Using an arbitrarily-primed polymerase chain reaction, 6 distinct C. difficile isolates were detected in the feces of the 9 affected horses at the time of the outbreak of diarrhea.
AB - Intestinal colonization with toxigenic strains of Clostridium difficile was documented in 9 of 10 horses with acute onset diarrhea in a veterinary medical teaching hospital, whereas a similar isolate was detected in only 1 of 23 other horses without diarrhea in the hospital. One horse with diarrhea was infected simultaneously with both C. difficile and Salmonella krefeld. Clostridium difficile was detected by fecal culture on selective medium, confirmed with a latex particle agglutination test, and identified as toxigenic by polymerase chain reaction amplification of toxin A and toxin B gene sequences. Using an arbitrarily-primed polymerase chain reaction, 6 distinct C. difficile isolates were detected in the feces of the 9 affected horses at the time of the outbreak of diarrhea.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029336929&partnerID=8YFLogxK
M3 - Article
C2 - 7578449
AN - SCOPUS:0029336929
VL - 7
SP - 343
EP - 346
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 3
ER -