Apoptosis occurs in the oligodendroglial lineage, and is prevented by basic fibroblast growth factor

T. Yasuda, J. Grinspan, J. Stern, B. Franceschini, P. Bannerman, David E Pleasure

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

During the perinatal period, oligodendroglial precursor cells proliferate rapidly, then cease dividing and differentiate into oligodendroglia. Many of these newly formed oligodendroglia are destined to die. We now demonstrate that oligodendroglia generated in passaged cultures of rat forebrain oligodendroglial precursor cells after removal of basic fibroblast growth factor (basic FGF) from the medium often undergo internucleosomal DNA nicking and nuclear fragmentation, features characteristic of apoptosis. These alterations are rare in cultures maintained continuously in basic FGF. As in many other cellular lineages susceptible to apoptosis, these degenerative changes can be prevented by treatment with the endonuclease antagonist, aurintricarboxylic acid, or by inhibiting de novo RNA or protein synthesis. Supplementation of the basic FGF-free medium with insulin, insulin-like growth factor-1, platelet-derived growth factor, or ciliary neuronotrophic growth factor also diminishes DNA nicking. Both oligodendroglial differentiation and DNA nicking are induced in basic FGF-treated cultures by inhibiting DNA synthesis with aphidicholin or excess thymidine, thus suggesting a close linkage between the anti-apoptotic, anti-differentiation, and mitogenic effects of basic FGF on the oligodendroglial lineage.

Original languageEnglish (US)
Pages (from-to)306-317
Number of pages12
JournalJournal of Neuroscience Research
Volume40
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Apoptosis
Oligodendroglia
DNA
Aurintricarboxylic Acid
Ciliary Neurotrophic Factor
Endonucleases
Platelet-Derived Growth Factor
Somatomedins
Prosencephalon
Thymidine
Intercellular Signaling Peptides and Proteins
RNA
Insulin
Proteins

Keywords

  • apoptosis
  • basic fibroblast growth factor
  • cell cycle
  • insulin-like growth factor-1
  • oligodendroglia
  • programmed cell death

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Apoptosis occurs in the oligodendroglial lineage, and is prevented by basic fibroblast growth factor. / Yasuda, T.; Grinspan, J.; Stern, J.; Franceschini, B.; Bannerman, P.; Pleasure, David E.

In: Journal of Neuroscience Research, Vol. 40, No. 3, 1995, p. 306-317.

Research output: Contribution to journalArticle

Yasuda, T. ; Grinspan, J. ; Stern, J. ; Franceschini, B. ; Bannerman, P. ; Pleasure, David E. / Apoptosis occurs in the oligodendroglial lineage, and is prevented by basic fibroblast growth factor. In: Journal of Neuroscience Research. 1995 ; Vol. 40, No. 3. pp. 306-317.
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