Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome

C. Wójcik, T. Stoklosa, Adam Giermasz, J. Golab, R. Zagozdzon, J. Kawiak, S. Wilk, A. Komar, A. Kaca, J. Malejczyk, M. Jakóbisiak

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Of a number of factors involved in apoptosis, protease activity may play a crucial role. We show that N-benzyloxycarbonyl-IIe-Glu(O-t-butyl)-Ala-leucinal (PSI), a selective inhibitor of the chymotrypsin-like activity of the proteasome, induces massive apoptosis in murine leukaemia L1210 cells. At 50 nM concentration, PSI induces a block of cytokinesis, while higher concentrations (500 nM) cause S phase block and massive apoptosis. Z-Leu-leucinal, a specific calpain inhibitor, did not induce apoptosis. In contrast to previous reports, TNF-α did not enhance apoptosis when combined with PSI. Our results suggest that proteasome inhibitors may be considered as potential anti-neoplastic agents.

Original languageEnglish (US)
Pages (from-to)455-462
Number of pages8
JournalApoptosis
Volume2
Issue number5
StatePublished - 1997
Externally publishedYes

Keywords

  • Apoptosis
  • Experimental cancer therapy
  • L1210 leukaemia
  • Proteasome, proteasome inhibitor, TNF

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Cancer Research

Fingerprint Dive into the research topics of 'Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome'. Together they form a unique fingerprint.

  • Cite this

    Wójcik, C., Stoklosa, T., Giermasz, A., Golab, J., Zagozdzon, R., Kawiak, J., Wilk, S., Komar, A., Kaca, A., Malejczyk, J., & Jakóbisiak, M. (1997). Apoptosis induced in L1210 leukaemia cells by an inhibitor of the chymotrypsin-like activity of the proteasome. Apoptosis, 2(5), 455-462.