Apolipoprotein E polymorphism in a healthy Swedish population: Variation of allele frequency with age and relation to serum lipid concentrations

Gösta Eggertsen, Ragnar Tegelman, Sverker Ericsson, Bo Angelin, Lars Berglund

Research output: Contribution to journalArticle

130 Scopus citations

Abstract

We analyzed blood samples from 407 healthy Swedish individuals, 244 men and 163 women, ages 17 to 86 years, for apolipoprotein (apo) E isoforms and serum triglycerides, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and total cholesterol. Parallel genotyping by means of polymerase chain reaction (PCR)-amplified DNA was performed in 200 subjects. Identical results were obtained by genotyping and phenotyping in 95% of all subjects analyzed. The apo E allelic frequencies were 7.8% for ∈2, 71.9% for ∈3, and 20.3% for ∈4. Compared with other Caucasian populations, the present population had a high relative allelic frequency of e4. The ∈4 frequency decreased with increasing age and was significantly lower in individuals >60 years of age (14.7%). When controlling for age and sex, there were strong correlations between total serum and LDL cholesterol and the various ∈ alleles. The ∈4 and ∈3 alleles correlated positively with serum cholesterol and the ∈4 allele correlated positively with LDL cholesterol. In contrast, HDL cholesterol and serum triglycerides did not show any correlation to the allele types. Thus, the results demonstrate a considerable age variation of the ∈ allele frequency among healthy Swedes and an influence of apo E alleles on serum and LDL cholesterol concentrations.

Original languageEnglish (US)
Pages (from-to)2125-2129
Number of pages5
JournalClinical Chemistry
Volume39
Issue number10
StatePublished - 1993
Externally publishedYes

Keywords

  • Isoelectric focusing
  • Lipoproteins
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Clinical Biochemistry

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