Apolipoprotein E-immunoreactivity in aged rhesus monkey cortex: Colocalization with amyloid plaques

Elliott J. Mufson, William C. Benzing, Greg M. Cole, Hua Wang, Dwaine F. Emerich, John R. Sladek, John Morrison, Jeffrey H. Kordower

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

In the present study, we examined the relationship between ApoE and amyloid containing profiles within the cerebral cortex of young, middle aged, and aged Rhesus monkeys. Polymerase chain reaction analysis revealed a pattern consistent with the ApoE e4 phenotype in the rhesus monkey similar to that reported in humans. We found numerous ApoE immunoreactive plaques within the temporal neocortex and amygdala, whereas the hippocampus contained only a few such plaques. Although virtually all ApoE-immunoreactive plaques coexpressed β-amyloid, most plaques were βA4 positive/ApoE immunonegative within the aged monkey cortex. Moreover, we observed a close correspondence between ApoE and thioflavin-positive (i.e., amyloid) plaques suggesting that ApoE may play a critical role in the conversion of βA4 to its β-pleated form. Because ApoE, βA4 and amyloid are expressed in plaques within the aged Rhesus macaque cortex, this species may provide an in vivo model for investigations aimed at clarifying the interactions between these proteins in normal and pathologic aging.

Original languageEnglish (US)
Pages (from-to)621-627
Number of pages7
JournalNeurobiology of Aging
Volume15
Issue number5
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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Keywords

  • Aging
  • Alzheimer's disease
  • Amyloid
  • Animal model
  • Antibodies
  • ApoE
  • Apolipoprotein
  • Immunohistochemistry
  • Monkey
  • Pathologic
  • Plaques
  • Thioflavin-S

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Developmental Biology
  • Geriatrics and Gerontology
  • Clinical Neurology

Cite this

Mufson, E. J., Benzing, W. C., Cole, G. M., Wang, H., Emerich, D. F., Sladek, J. R., Morrison, J., & Kordower, J. H. (1994). Apolipoprotein E-immunoreactivity in aged rhesus monkey cortex: Colocalization with amyloid plaques. Neurobiology of Aging, 15(5), 621-627. https://doi.org/10.1016/0197-4580(94)00064-6