Apolipoprotein B synthesis in humans: Liver synthesizes only apolipoprotein B-100

Stephen B. Edge, Jeffrey M. Hoeg, Philip D Schneider, H. Bryan Brewer

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Apolipoprotein (apo) B-100 and B-48 are prominent apolipoprotein in VLDL, IDL, and chylomicrons. Organ cultures of normal adult human liver were established to ascertain the form of apo B synthesized by hepatocytes in humans. Human liver was minced and incubated in 15 mL methionine-free RPMI-1640 medium with 10% dialyzed fetal calf serum plus 250 μCi 35S-methionine for eight hours at 37 °C. Lipoproteins secreted by the liver were isolated by ultracentrifugation and the content of newly synthesized apo B determined by quantitation of radioactivity in the apoB-100 and apoB-48 bands after separation by 3% NaDodSO4 gel electrophoresis. In the eight-hour period, 2.5% to 3.2% of added 35S-methionine was secreted in TCA-precipitable protein of which 0.34% was apo B. Ninety-nine percent of the apo B in VLDL, IDL, and LDL was in the apo B-100 electrophoretic band. No significant radioactivity was detected in the apo B-48 electrophoretic band. Eighty-nine percent of the total radioactivity of apo B-100 was in VLDL with 3% and 8% in IDL and LDL, respectively. These results establish that adult human liver in organ culture synthesizes apo B-100 but not apo B-48.* * Published in abstract form for the American Heart Association Meeting, November 1983. Arteriosclerosis 3:499a, 1983.

Original languageEnglish (US)
Pages (from-to)726-730
Number of pages5
JournalMetabolism
Volume34
Issue number8
DOIs
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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