TY - JOUR
T1 - Apolipoprotein B synthesis in humans
T2 - Liver synthesizes only apolipoprotein B-100
AU - Edge, Stephen B.
AU - Hoeg, Jeffrey M.
AU - Schneider, Philip D
AU - Brewer, H. Bryan
PY - 1985
Y1 - 1985
N2 - Apolipoprotein (apo) B-100 and B-48 are prominent apolipoprotein in VLDL, IDL, and chylomicrons. Organ cultures of normal adult human liver were established to ascertain the form of apo B synthesized by hepatocytes in humans. Human liver was minced and incubated in 15 mL methionine-free RPMI-1640 medium with 10% dialyzed fetal calf serum plus 250 μCi 35S-methionine for eight hours at 37 °C. Lipoproteins secreted by the liver were isolated by ultracentrifugation and the content of newly synthesized apo B determined by quantitation of radioactivity in the apoB-100 and apoB-48 bands after separation by 3% NaDodSO4 gel electrophoresis. In the eight-hour period, 2.5% to 3.2% of added 35S-methionine was secreted in TCA-precipitable protein of which 0.34% was apo B. Ninety-nine percent of the apo B in VLDL, IDL, and LDL was in the apo B-100 electrophoretic band. No significant radioactivity was detected in the apo B-48 electrophoretic band. Eighty-nine percent of the total radioactivity of apo B-100 was in VLDL with 3% and 8% in IDL and LDL, respectively. These results establish that adult human liver in organ culture synthesizes apo B-100 but not apo B-48.* * Published in abstract form for the American Heart Association Meeting, November 1983. Arteriosclerosis 3:499a, 1983.
AB - Apolipoprotein (apo) B-100 and B-48 are prominent apolipoprotein in VLDL, IDL, and chylomicrons. Organ cultures of normal adult human liver were established to ascertain the form of apo B synthesized by hepatocytes in humans. Human liver was minced and incubated in 15 mL methionine-free RPMI-1640 medium with 10% dialyzed fetal calf serum plus 250 μCi 35S-methionine for eight hours at 37 °C. Lipoproteins secreted by the liver were isolated by ultracentrifugation and the content of newly synthesized apo B determined by quantitation of radioactivity in the apoB-100 and apoB-48 bands after separation by 3% NaDodSO4 gel electrophoresis. In the eight-hour period, 2.5% to 3.2% of added 35S-methionine was secreted in TCA-precipitable protein of which 0.34% was apo B. Ninety-nine percent of the apo B in VLDL, IDL, and LDL was in the apo B-100 electrophoretic band. No significant radioactivity was detected in the apo B-48 electrophoretic band. Eighty-nine percent of the total radioactivity of apo B-100 was in VLDL with 3% and 8% in IDL and LDL, respectively. These results establish that adult human liver in organ culture synthesizes apo B-100 but not apo B-48.* * Published in abstract form for the American Heart Association Meeting, November 1983. Arteriosclerosis 3:499a, 1983.
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U2 - 10.1016/0026-0495(85)90022-8
DO - 10.1016/0026-0495(85)90022-8
M3 - Article
C2 - 4021804
AN - SCOPUS:0021800756
VL - 34
SP - 726
EP - 730
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
SN - 0026-0495
IS - 8
ER -