AP2-dependent signals from the ectoderm regulate craniofacial development in the zebrafish embryo

Robert D. Knight, Yashar Javidan, Tailin Zhang, Sarah Nelson, Thomas F. Schilling

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

AP2 transcription factors regulate many aspects of embryonic development. Studies of AP2a (Tfap2a) function in mice and zebrafish have demonstrated a role in patterning mesenchymal cells of neural crest origin that form the craniofacial skeleton, while the mammalian Tfap2b is required in both the facial skeleton and kidney. Here, we show essential functions for zebrafish tfap2a and tfap2b in development of the facial ectoderm, and for signals from this epithelium that induce skeletogenesis in neural crest cells (NCCs). Zebrafish embryos deficient for both tfap2a and tfap2b show defects in epidermal cell survival and lack NCC-derived cartilages. We show that cartilage defects arise after NCC migration during skeletal differentiation, and that they can be rescued by transplantation of wild-type ectoderm. We propose a model in which AP2 proteins play two distinct roles in cranial NCCs: an early cell-autonomous function in cell specification and survival, and a later non-autonomous function regulating ectodermal signals that induce skeletogenesis.

Original languageEnglish (US)
Pages (from-to)3127-3138
Number of pages12
JournalDevelopment
Volume132
Issue number13
DOIs
StatePublished - Jul 1 2005
Externally publishedYes

Fingerprint

Ectoderm
Neural Crest
Zebrafish
Embryonic Structures
Skeleton
Cartilage
Cell Survival
Transcription Factor AP-2
Embryonic Development
Cell Movement
Epithelium
Transplantation
Kidney
Proteins

Keywords

  • Craniofacial
  • Danio rerio
  • Neural crest
  • Tcfap2

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

AP2-dependent signals from the ectoderm regulate craniofacial development in the zebrafish embryo. / Knight, Robert D.; Javidan, Yashar; Zhang, Tailin; Nelson, Sarah; Schilling, Thomas F.

In: Development, Vol. 132, No. 13, 01.07.2005, p. 3127-3138.

Research output: Contribution to journalArticle

Knight, Robert D. ; Javidan, Yashar ; Zhang, Tailin ; Nelson, Sarah ; Schilling, Thomas F. / AP2-dependent signals from the ectoderm regulate craniofacial development in the zebrafish embryo. In: Development. 2005 ; Vol. 132, No. 13. pp. 3127-3138.
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