Aortic antioxidant enzyme activity in human aneurysmal and occlusive disease

M. A. Dubick, Carl L Keen, R. A. Disilvestro, C. D. Eskelsnn, G. C. Hunter

Research output: Contribution to journalArticlepeer-review

Abstract

The present study further investigates evidence for lipid peroxidation in atherosclerotic aortic tissue by determining the activity of antioxidant enzymes and concentrations of lipid peroxide fluorochromes in abdominal aortas from 15 patients with aortic aneurysms (AA) and 12 patients with atherosclerotic occlusive disease (OD). Aortas from nonatherosclerotic organ donors served as controls. In both AA and OD aortas, total Superoxide dismutase (SOD) activity was less than 50% of control activity. Further analysis of these tissues showed that Mn-SOD activity was 61% and Cu,Zn-SOD activity was 25% and 38% of control activity. CuZn-SOD protein in AA and OD was 56% and 100% of controls, respectively, resulting in significantly lower CuZn-SOD specific activity in these tissues. Glutathione peroxidase activity in AA and OD aortas was 63% and 51 % of controls, respectively, while glutathione reductase activity in AA and OD aortas was 80% and 59% of control activity. These results were associated with significantly higher lipid peroxide fluorochromes, expressed as U/g aorta, in both groups of atherosclerotic aortas than in controls. OD aortas also had 33% higher fluorescence than AA aortas. These data further support the involvement of free radicals and lipid peroxidation in atheroscleroticaortic disease.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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