Antitumor effect of a novel adeno-associated virus vector targeting to telomerase activity in tumor cells

Yi Gang Wang, Jin Hui Wang, Yan Hong Zhang, Qing Gu, Xin Yuan Liu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Telomerase activity is a wide tumor marker. Human telomerase reverse transcriptase (hTERT), the catalytic subunit of the telomerase, is transcriptionally upregulated exclusively in about 90% of cancer cells. In this study, we constructed a novel adeno-associated virus (AAV) vector containing the human interferon-β (hIFN-β) gene under the control of hTERT promoter (AAV-hTERT-hIFN-β) and investigated its antitumor effect against various human cancer cells in vitro. AAV-hTERT-hIFN-β displayed cancer-specific hIFN-β expression and cytotoxicity. The cytotoxic ratio was positively correlated with the time length of infection. AAV-hTERT-hIFN-β-mediated apoptotic morphology was observed by transmission electron microscopy. Flow cytometry assay also revealed that the cytotoxicity of AAV-hTERT-hIFN-β was mainly an apoptotic process. These data indicate that AAV in combination with hTERT-mediated therapeutic gene expression may open new possibilities for long-lasting and targeting gene therapy of varieties of cancers.

Original languageEnglish (US)
Pages (from-to)492-500
Number of pages9
JournalActa Biochimica et Biophysica Sinica
Volume36
Issue number7
DOIs
StatePublished - Jul 2004
Externally publishedYes

Keywords

  • Adeno-associated virus (AAV)
  • Cancer
  • hTERT promoter
  • Human interferon-β
  • Telomerase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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